BRENDA - Enzyme Database show

Expression and activity of steroid sulphatase in the boar testis

Mutembei, H.M.; Kowalewski, M.P.; Ugele, B.; Schuler, G.; Hoffmann, B.; Reprod. Domest. Anim. 44, 17-23 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.6.2
partial sequence of porcine StS, subcloned into pGEM T vector, amplified in Escherichia coli
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.2
0.00215
-
Estrone sulfate
37°C, 10 min reaction time, Ringer-HEPES buffer, Vmax=24.05 fmol/s/microgram protein
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.6.2
cytoplasm
-
Sus scrofa
5737
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.2
estrone sulfate + H2O
Sus scrofa
STS within the Leydig cells of Sus scrofa is involved in modulation of testicular estrogen bioavailability
estrone + sulfate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.6.2
Sus scrofa
Q45FD5
Sus scrofa steroid sulfatase (StS) mRNA, partial cds from boar testis; 5 age groups 50, 100, 150, 250 days old boars, remarkably high testicular estrogen output
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.2
Leydig cell
all cells with positive StS expression,exception: 65.1% StS expression in 50-day-old animal's Leydig cells
Sus scrofa
-
3.1.6.2
testis
-
Sus scrofa
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.2
estrone sulfate + H2O
-
695080
Sus scrofa
estrone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
STS within the Leydig cells of Sus scrofa is involved in modulation of testicular estrogen bioavailability
695080
Sus scrofa
estrone + sulfate
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.6.2
partial sequence of porcine StS, subcloned into pGEM T vector, amplified in Escherichia coli
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.2
0.00215
-
Estrone sulfate
37°C, 10 min reaction time, Ringer-HEPES buffer, Vmax=24.05 fmol/s/microgram protein
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.6.2
cytoplasm
-
Sus scrofa
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.2
estrone sulfate + H2O
Sus scrofa
STS within the Leydig cells of Sus scrofa is involved in modulation of testicular estrogen bioavailability
estrone + sulfate
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.2
Leydig cell
all cells with positive StS expression,exception: 65.1% StS expression in 50-day-old animal's Leydig cells
Sus scrofa
-
3.1.6.2
testis
-
Sus scrofa
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.2
estrone sulfate + H2O
-
695080
Sus scrofa
estrone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
STS within the Leydig cells of Sus scrofa is involved in modulation of testicular estrogen bioavailability
695080
Sus scrofa
estrone + sulfate
-
-
-
?