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Literature summary extracted from
- Kinetic and structural properties of triosephosphate isomerase from Helicobacter pylori (2008), Proteins Struct. Funct. Bioinform., 71, 396-406.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.1 | - |
Helicobacter pylori |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.3.1.1 | monomer, to 2.3 A resolution | Helicobacter pylori |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.3.1.1 | D213A | disruption of the salt bridge between residues D213 and K183, kinetic parameters similar to wild-type | Helicobacter pylori |
| 5.3.1.1 | D213Q | disruption of the salt bridge between residues D213 and K183, kinetic parameters similar to wild-type | Helicobacter pylori |
| 5.3.1.1 | K183A | disruption of the salt bridge between residues D213 and K183, kinetic parameters similar to wild-type | Helicobacter pylori |
| 5.3.1.1 | K183S | disruption of the salt bridge between residues D213 and K183, kinetic parameters similar to wild-type | Helicobacter pylori |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.1 | 2.49 | - |
D-glyceraldehyde 3-phosphate | mutant D213A, pH 7.5, 25°C | Helicobacter pylori |  |
| 5.3.1.1 | 3.27 | - |
D-glyceraldehyde 3-phosphate | mutant D213Q, pH 7.5, 25°C | Helicobacter pylori | |
| 5.3.1.1 | 3.46 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.5, 25°C | Helicobacter pylori | |
| 5.3.1.1 | 3.56 | - |
D-glyceraldehyde 3-phosphate | mutant K183S, pH 7.5, 25°C | Helicobacter pylori | |
| 5.3.1.1 | 9.09 | - |
D-glyceraldehyde 3-phosphate | mutant K183A, pH 7.5, 25°C | Helicobacter pylori | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.1 | 28108 | - |
2 * 28108, mass spectrometry | Helicobacter pylori |
| 5.3.1.1 | 56000 | - |
gel filtration | Helicobacter pylori |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.1 | Helicobacter pylori | P56076 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.1 | D-glyceraldehyde 3-phosphate | - |
Helicobacter pylori | dihydroxyacetone phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.1 | dimer | 2 * 28108, mass spectrometry | Helicobacter pylori |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.1 | 14670 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.5, 25°C | Helicobacter pylori | |
| 5.3.1.1 | 15670 | - |
D-glyceraldehyde 3-phosphate | mutant D213A, pH 7.5, 25°C | Helicobacter pylori | |
| 5.3.1.1 | 18330 | - |
D-glyceraldehyde 3-phosphate | mutant D213Q, pH 7.5, 25°C | Helicobacter pylori | |
| 5.3.1.1 | 20000 | - |
D-glyceraldehyde 3-phosphate | mutant K183S, pH 7.5, 25°C | Helicobacter pylori | |
| 5.3.1.1 | 23330 | - |
D-glyceraldehyde 3-phosphate | mutant K183A, pH 7.5, 25°C | Helicobacter pylori | |
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