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Literature summary extracted from

  • Pelegrini, P.B.; Lay, F.T.; Murad, A.M.; Anderson, M.A.; Franco, O.L.
    Novel insights on the mechanism of action of alpha-amylase inhibitors from the plant defensin family (2008), Proteins, 73, 719-729.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 additional information Vigna unguiculata defensin i.e. VuD1, plant defensins are small protein consisting of 45-54 amino acids, no inhibition with 0.1 mg/ml inhibitor, pH 6.5, 37°C Aspergillus fumigatus
3.2.1.1 Vigna unguiculata defensin i.e. VuD1, plant defensins are small protein consisting of 45-54 amino acids, below 62% inhibition with 0.1 mg/ml inhibitor, pH 6.5, 37°C Acanthoscelides obtectus
3.2.1.1 Vigna unguiculata defensin i.e. VuD1, plant defensins are small protein consisting of 45-54 amino acids, below 5% inhibition with 0.1 mg/ml inhibitor, pH 6.5, 37°C Callosobruchus maculatus
3.2.1.1 Vigna unguiculata defensin i.e. VuD1, plant defensins are small protein consisting of 45-54 amino acids, ca. 38% inhibition with 0.1 mg/ml inhibitor, pH 6.5, 37°C Homo sapiens
3.2.1.1 Vigna unguiculata defensin i.e. VuD1, plant defensins are small protein consisting of 45-54 amino acids, ca. 12% inhibition with 0.1 mg/ml inhibitor, pH 6.5, 37°C, low inhibition activity in pig is probably related to extended loops in the structural core of the amylase, reducing the contact between defensin VuD1 and the catalytic site Sus scrofa
3.2.1.1 Vigna unguiculata defensin i.e. VuD1, plant defensins are small protein consisting of 45-54 amino acids, below 60% inhibition with 0.1 mg/ml inhibitor, pH 6.5, 37°C, Lys1 from the defensin VuD1 appears to interact with residue Asp204 from the anylase ZSA, which is located inside the catalytic site, analyzed by in silico docking experiments Zabrotes subfasciatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Acanthoscelides obtectus
-
 bean weevil
-
3.2.1.1 Aspergillus fumigatus
-
-
-
3.2.1.1 Callosobruchus maculatus
-
-
-
3.2.1.1 Homo sapiens
-
-
-
3.2.1.1 Sus scrofa P00690
-
-
3.2.1.1 Zabrotes subfasciatus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.1 larva
-
 Zabrotes subfasciatus
-
3.2.1.1 larva
-
 Acanthoscelides obtectus
-
3.2.1.1 larva
-
 Callosobruchus maculatus
-
3.2.1.1 midgut
-
 Zabrotes subfasciatus
-
3.2.1.1 midgut
-
 Acanthoscelides obtectus
-
3.2.1.1 midgut
-
 Callosobruchus maculatus
-
3.2.1.1 pancreas
-
 Sus scrofa
-
3.2.1.1 saliva
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 starch + H2O
-
 Homo sapiens ?
-
 ?
3.2.1.1 starch + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.1 starch + H2O pH 6.5, 37°C Zabrotes subfasciatus ?
-
 ?
3.2.1.1 starch + H2O pH 6.5, 37°C Acanthoscelides obtectus ?
-
 ?
3.2.1.1 starch + H2O pH 6.5, 37°C Callosobruchus maculatus ?
-
 ?
3.2.1.1 starch + H2O pH 6.5, 37°C Sus scrofa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 alpha-amylase Aasp
-
 Aspergillus fumigatus
3.2.1.1 alpha-amylase AOA
-
 Acanthoscelides obtectus
3.2.1.1 alpha-amylase CMA
-
 Callosobruchus maculatus
3.2.1.1 alpha-amylase HA
-
 Homo sapiens
3.2.1.1 alpha-amylase PPA
-
 Sus scrofa
3.2.1.1 alpha-amylase ZSA
-
 Zabrotes subfasciatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 37
-
 assay at Homo sapiens
3.2.1.1 37
-
 assay at Aspergillus fumigatus
3.2.1.1 37
-
 assay at Zabrotes subfasciatus
3.2.1.1 37
-
 assay at Acanthoscelides obtectus
3.2.1.1 37
-
 assay at Callosobruchus maculatus
3.2.1.1 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 6.5
-
 assay at Homo sapiens
3.2.1.1 6.5
-
 assay at Aspergillus fumigatus
3.2.1.1 6.5
-
 assay at Zabrotes subfasciatus
3.2.1.1 6.5
-
 assay at Acanthoscelides obtectus
3.2.1.1 6.5
-
 assay at Callosobruchus maculatus
3.2.1.1 6.5
-
 assay at Sus scrofa