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Literature summary extracted from

  • Antranikian, G.; Herzberg, C.; Gottschalk, G.
    Covalent modification of citrate lyase ligase from Clostridium sphenoides by phosphorylation/dephosphorylation (1985), Eur. J. Biochem., 153, 413-420.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.22 AMP
-
 Lacrimispora sphenoides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.22 0.4
-
 ATP
-
 Lacrimispora sphenoides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.22 Mg2+ optimal Mg2+ concentration: 1.6-1.9 mM Lacrimispora sphenoides
6.2.1.22 PO43- activity is modulated by phosphorylation/dephosphorylation, active form of enzyme is phosphorylated Lacrimispora sphenoides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.22 56000
-
 1 * 56000, SDS-PAGE Lacrimispora sphenoides
6.2.1.22 58000
-
 gel filtration, sucrose density gradient centrifugation Lacrimispora sphenoides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.22 ATP + acetate + citrate (pro-3S)-lyase Lacrimispora sphenoides thiol form of (pro-3S)-lyase, enzyme converts the inactive thiol form of EC 4.1.3.6 into the active form. EC 4.1.3.6 is the enzyme responsible for the anaerobic utilization of citrate ?
-
 ?
6.2.1.22 ATP + acetate + citrate (pro-3S)-lyase Lacrimispora sphenoides C2 / DSM 614 thiol form of (pro-3S)-lyase, enzyme converts the inactive thiol form of EC 4.1.3.6 into the active form. EC 4.1.3.6 is the enzyme responsible for the anaerobic utilization of citrate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.22 Lacrimispora sphenoides
-
-
-
6.2.1.22 Lacrimispora sphenoides C2 / DSM 614
-
-
-
6.2.1.22 Lactococcus lactis
-
-
-
6.2.1.22 [Clostridium] sporosphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.22
-
 Lacrimispora sphenoides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.22 additional information
-
-
 Lacrimispora sphenoides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.22 ATP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase Lacrimispora sphenoides AMP + diphosphate + citrate(pro-3S)-lyase
-
 ?
6.2.1.22 ATP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase [Clostridium] sporosphaeroides AMP + diphosphate + citrate(pro-3S)-lyase
-
 ?
6.2.1.22 ATP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase Lactococcus lactis AMP + diphosphate + citrate(pro-3S)-lyase
-
 ?
6.2.1.22 ATP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase Lacrimispora sphenoides C2 / DSM 614 AMP + diphosphate + citrate(pro-3S)-lyase
-
 ?
6.2.1.22 ATP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase, enzyme converts the inactive thiol form of EC 4.1.3.6 into the active form. EC 4.1.3.6 is the enzyme responsible for the anaerobic utilization of citrate Lacrimispora sphenoides ?
-
 ?
6.2.1.22 ATP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase, enzyme converts the inactive thiol form of EC 4.1.3.6 into the active form. EC 4.1.3.6 is the enzyme responsible for the anaerobic utilization of citrate Lacrimispora sphenoides C2 / DSM 614 ?
-
 ?
6.2.1.22 dATP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase, dATP at 72% of the activity relative to ATP Lacrimispora sphenoides dAMP + diphosphate + citrate(pro-3S)-lyase
-
 ?
6.2.1.22 dATP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase, dATP at 72% of the activity relative to ATP Lacrimispora sphenoides C2 / DSM 614 dAMP + diphosphate + citrate(pro-3S)-lyase
-
 ?
6.2.1.22 ITP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase, ITP at 33% of the activity relative to ATP Lacrimispora sphenoides IMP + diphosphate + citrate(pro-3S)-lyase
-
 ?
6.2.1.22 ITP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase, ITP at 33% of the activity relative to ATP Lacrimispora sphenoides C2 / DSM 614 IMP + diphosphate + citrate(pro-3S)-lyase
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.22 monomer 1 * 56000, SDS-PAGE Lacrimispora sphenoides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.22 7.6
-
-
 Lacrimispora sphenoides