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Literature summary extracted from

  • Cheung, A.H.; Wong, J.H.; Ng, T.B.
    Trypsin-chymotrypsin inhibitors from Vigna mungo seeds (2009), Protein Pept. Lett., 16, 277-284.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.1 BGTI1 concentration 228 nM: 100% inhibition, concentration 456 nM: 97.8% inhibition, concentration 114 nM: 73.3% inhibition, concentration 57 nM: 41.3% inhibition. Inhibitor from Vigna mungo seeds Bos taurus
3.4.21.1 BGTI2 concentration 2188 nM: 53.3% inhibition, concentration 1094 nM: 28.9% inhibition, concentration 547 nM: 11.1% inhibition, concentration 273 nM: 6.7% inhibition. Inhibitor from Vigna mungo seeds Bos taurus
3.4.21.1 BGTI3 concentration 5000 nM: 36.7% inhibition, concentration 2500 nM: 22.3% inhibition, concentration 1250 nM: 10.0% inhibition, concentration 625 nM: 0.0% inhibition. Inhibitor from Vigna mungo seeds Bos taurus
3.4.21.1 additional information protease inhibitor does not exert any inhibitory effect on hepatoma (Hep G2) and breast cancer (MCF 7) cells or antifungal action toward Botrytis cinerea, Fusarium oxysporum and Mycosphaerella arachidicola Bos taurus
3.4.21.4 black gram trypsin inhibitor 1 highly effective trypsin inhibitor, 87.5% of trypsin inhibitory activity of black gram trypsin inhibitor 1 is retained after treatment with 10 mM dithiothreitol for 2 hours, and the activity dwindles to 12.5% after treatment with 100 mM dithiothreitol for 2 hours Bos taurus
3.4.21.4 black gram trypsin inhibitor 2 highly effective trypsin inhibitor, the trypsin inhibitory activity of black gram trypsin inhibitor 2 is unaffected after exposure to 100 mM dithiothreitol for 2 hours Bos taurus
3.4.21.4 black gram trypsin inhibitor 3 highly effective trypsin inhibitor, in the presence of 10 mM dithiothreitol trypsin inhibitory of black gram trypsin inhibitor 3 drops to 57.1% after 1 h and becomes undetectable after 2 hours, while treatment of 100 mM dithiothreitol for 1 h has the same effect Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.1 Bos taurus
-
-
-
3.4.21.4 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.4 pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.1 N-alpha-benzoyl-L-tyrosine ethyl ester + H2O
-
 Bos taurus ?
-
 ?
3.4.21.4 N-alpha-benzoyl-L-Arg-ethyl ester + H2O
-
 Bos taurus N-alpha-benzoyl-L-Arg + ethanol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.1 chymotrypsin
-
 Bos taurus