Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Le, D.; Hua, X.; Huang, L.; Gao, G.; Lu, H.; Xu, Z.; Tian, B.; Hua, Y.
    Biochemical characterization of two DNA ligases from Deinococcus radiodurans (2008), Protein Pept. Lett., 15, 600-605.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expressed in Escherichia coli Deinococcus radiodurans
6.5.1.2 expressed in Escherichia coli Deinococcus radiodurans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Mg2+ optimum concentration at 5 mM Deinococcus radiodurans
6.5.1.2 Mg2+ optimum concentration at 5 mM Deinococcus radiodurans

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Deinococcus radiodurans
-
-
-
6.5.1.2 Deinococcus radiodurans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1
-
 Deinococcus radiodurans
6.5.1.2
-
 Deinococcus radiodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Deinococcus radiodurans AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Deinococcus radiodurans AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 ATP-dependent DNA ligase
-
 Deinococcus radiodurans
6.5.1.1 LigB
-
 Deinococcus radiodurans
6.5.1.2 LigA
-
 Deinococcus radiodurans
6.5.1.2 NAD(+)-dependent DNA ligase
-
 Deinococcus radiodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.1 60
-
-
 Deinococcus radiodurans
6.5.1.2 60
-
-
 Deinococcus radiodurans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.5.1.1 100
-
 the half-life of heat inactivation at 100°C is about 5 min Deinococcus radiodurans
6.5.1.2 100
-
 the half-life of heat inactivation at 100°C is about 3 min Deinococcus radiodurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.1 7
-
-
 Deinococcus radiodurans
6.5.1.2 7
-
-
 Deinococcus radiodurans

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.1 ATP dependent on Deinococcus radiodurans
6.5.1.2 NAD+ dependent on Deinococcus radiodurans