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Literature summary extracted from

  • Sato, R.; Matsumoto, T.; Hidaka, N.; Imai, Y.; Abe, K.; Takahashi, S.; Yamada, R.H.; Kera, Y.
    Cloning and expression of carp acetylcholinesterase gene in Pichia pastoris and characterization of the recombinant enzyme (2009), Protein Expr. Purif., 64, 205-212.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.7 expression analysis by semi-quantitative PCR, sequence comparisons, subcloning of carp AChE in Escherichia coli strain DH5alpha, and expression of wild-type and mutant enzymes in Pichia pastoris strain X-33 Cyprinus carpio

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.7 additional information construction of two mutant enzymes, one lacking the T peptide and the second lacking the T peptide and containing the alpha-factor prepro signal sequence from Saccharomyces cerevisiae in place of the original signal sequence Cyprinus carpio

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 acetylthiocholine the recombinant carp AChE shows substrate inhibition at high substrate concentrations of acetyl- and propionylthiocholine Cyprinus carpio
3.1.1.7 acetylthiocholine iodide
-
Cyprinus carpio
3.1.1.7 butyrylthiocholine
-
Cyprinus carpio
3.1.1.7 butyrylthiocholine iodide
-
Cyprinus carpio
3.1.1.7 BW284c51
-
Cyprinus carpio
3.1.1.7 diisopropylfluorophosphate
-
Cyprinus carpio
3.1.1.7 edrophonium
-
Cyprinus carpio
3.1.1.7 ethopropazine low inhibition Cyprinus carpio
3.1.1.7 Phenylmethylsulfonylfluoride
-
Cyprinus carpio
3.1.1.7 procainamide low inhibition Cyprinus carpio
3.1.1.7 propionylthiocholine the recombinant carp AChE shows substrate inhibition at high substrate concentrations of acetyl- and propionylthiocholine Cyprinus carpio

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.7 0.000232
-
acetylthiocholine iodide pH 7.6, 40°C, native enzyme Cyprinus carpio
3.1.1.7 0.00041
-
butyrylthiocholine iodide pH 7.6, 40°C, native enzyme Cyprinus carpio
3.1.1.7 0.000526
-
propionylthiocholine iodide pH 7.6, 40°C, native enzyme Cyprinus carpio
3.1.1.7 0.00205
-
acetylthiocholine iodide pH 7.6, 40°C, recombinant enzyme Cyprinus carpio
3.1.1.7 0.00605
-
propionylthiocholine iodide pH 7.6, 40°C, recombinant enzyme Cyprinus carpio
3.1.1.7 0.109
-
butyrylthiocholine iodide pH 7.6, 40°C, recombinant enzyme Cyprinus carpio

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.7 22000
-
x * 73000 + x * 54000 + x * 22000, recombinant enzyme, SDS-PAGE Cyprinus carpio
3.1.1.7 54000
-
x * 73000 + x * 54000 + x * 22000, recombinant enzyme, SDS-PAGE Cyprinus carpio
3.1.1.7 73000
-
x * 73000 + x * 54000 + x * 22000, recombinant enzyme, SDS-PAGE Cyprinus carpio

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Cyprinus carpio B7X755
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.7 glycoprotein the enzyme is N-glycosylated, and the sequence contains N-glycosylation consensus sites Cyprinus carpio

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.7 recombinant wild-type and mutant enzymes 276fold from Pichia pastoris strain X-33 culture supernatant by anion exchange and edrophonium adsorption chromatography, followed by gel filtration Cyprinus carpio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.7 muscle
-
Cyprinus carpio
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.7 42
-
mutant enzyme lacking the T peptide and possessing a Saccharomyces cerevisiae alpha-factor prepro signal sequence in recombinant Pichia pastoris cells Cyprinus carpio
3.1.1.7 176
-
wild-type enzyme in recombinant Pichia pastoris cells Cyprinus carpio
3.1.1.7 181
-
mutant enzyme lacking the T peptide in recombinant Pichia pastoris cells Cyprinus carpio
3.1.1.7 4970
-
purified recombinant wild-type enzyme Cyprinus carpio

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 butyrylthiocholine iodide + H2O
-
Cyprinus carpio thiocholine iodide + butyrate
-
?
3.1.1.7 propionylthiocholine iodide + H2O
-
Cyprinus carpio thiocholine iodide + propionate
-
?
3.1.1.7 S-acetylthiocholine iodide + H2O detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB, the enzyme contains a Ser, Glu, and His catalytic triad, overview Cyprinus carpio acetate + thiocholine iodide
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.7 ? x * 73000 + x * 54000 + x * 22000, recombinant enzyme, SDS-PAGE Cyprinus carpio

Synonyms

EC Number Synonyms Comment Organism
3.1.1.7 AChE
-
Cyprinus carpio

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.7 40
-
-
Cyprinus carpio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.7 7.6
-
-
Cyprinus carpio

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.7 0.0056
-
acetylthiocholine iodide pH 7.6, 40°C, native enzyme Cyprinus carpio
3.1.1.7 0.175
-
acetylthiocholine iodide pH 7.6, 40°C, recombinant enzyme Cyprinus carpio
3.1.1.7 0.418
-
butyrylthiocholine iodide pH 7.6, 40°C, native enzyme Cyprinus carpio

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.7 0.00217
-
pH 7.6, 40°C, recombinant enzyme Cyprinus carpio ethopropazine
3.1.1.7 0.00294
-
pH 7.6, 40°C, recombinant enzyme Cyprinus carpio Phenylmethylsulfonylfluoride
3.1.1.7 0.00305
-
pH 7.6, 40°C, native enzyme Cyprinus carpio Phenylmethylsulfonylfluoride
3.1.1.7 0.00335
-
pH 7.6, 40°C, native enzyme Cyprinus carpio ethopropazine
3.1.1.7 0.00347
-
pH 7.6, 40°C, native enzyme Cyprinus carpio procainamide
3.1.1.7 0.00356
-
pH 7.6, 40°C, recombinant enzyme Cyprinus carpio procainamide
3.1.1.7 0.00439
-
pH 7.6, 40°C, recombinant enzyme Cyprinus carpio diisopropylfluorophosphate
3.1.1.7 0.00441
-
pH 7.6, 40°C, native enzyme Cyprinus carpio diisopropylfluorophosphate
3.1.1.7 0.00522
-
pH 7.6, 40°C, recombinant enzyme Cyprinus carpio edrophonium
3.1.1.7 0.0066
-
pH 7.6, 40°C, native enzyme Cyprinus carpio edrophonium
3.1.1.7 0.00682
-
pH 7.6, 40°C, recombinant enzyme Cyprinus carpio BW284c51
3.1.1.7 0.00722
-
pH 7.6, 40°C, native enzyme Cyprinus carpio BW284c51