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Literature summary extracted from

  • Wetterholm, A.; Martinez Molina, D.; Nordlund, P.; Eshaghi, S.; Haeggstroem, J.Z.
    High-level expression, purification, and crystallization of recombinant rat leukotriene C4 synthase from the yeast Pichia pastoris (2008), Protein Expr. Purif., 60, 1-6.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.20 expressed as an N-terminal hexa-histidine-tagged fusion protein in Pichia pastoris KM71H cells Rattus norvegicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.4.1.20 sitting drop vapour diffusion method using the MbClass and MbClass II suites Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.20 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.20 Ni-Sepharose column chromatography, S-hexylglutathione agarose chromatography, and Superdex 200 gel filtration Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.20 0.29
-
 crude enzyme from homogenate, at 25°C Rattus norvegicus
4.4.1.20 28
-
 recombinant enzyme, after 97fold purification, at 25°C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.20 leukotriene A4 + glutathione
-
 Rattus norvegicus leukotriene C4 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.20 leukotriene C4 synthase
-
 Rattus norvegicus
4.4.1.20 LTC4S
-
 Rattus norvegicus