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Literature summary extracted from

  • Ehren, J.; Govindarajan, S.; Moron, B.; Minshull, J.; Khosla, C.
    Protein engineering of improved prolyl endopeptidases for celiac sprue therapy (2008), Protein Eng. Des. Sel., 21, 699-707.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.26 additional information oral therapeutic candidate for protecting celiac sprue patients from the toxic effects of dietary gluten Novosphingobium capsulatum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.26 expressed in Escherichia coli Novosphingobium capsulatum

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.26 F227Y mutant of SC PEP Novosphingobium capsulatum
3.4.21.26 F459Y mutant of SC PEP Novosphingobium capsulatum
3.4.21.26 I371V mutant of SC PEP Novosphingobium capsulatum
3.4.21.26 I406V one of the mutations that shows the most significant correlation to improved activity and/or stability under simulated gastric conditions Novosphingobium capsulatum
3.4.21.26 I514V one of the mutations that shows the most significant correlation to improved activity and/or stability under simulated gastric conditions Novosphingobium capsulatum
3.4.21.26 I581V mutant of SC PEP Novosphingobium capsulatum
3.4.21.26 I687V mutant of SC PEP Novosphingobium capsulatum
3.4.21.26 K103T one of the mutations that shows the most significant correlation to improved activity and/or stability under simulated gastric conditions Novosphingobium capsulatum
3.4.21.26 M511L one of the mutations that shows the most significant correlation to improved activity and/or stability under simulated gastric conditions Novosphingobium capsulatum
3.4.21.26 additional information mutants of SC PEP, variants with as much as 20% enhanced specific activity at pH 4.5 and 200fold greater resistance to pepsin are identified Novosphingobium capsulatum
3.4.21.26 R100K mutant of SC PEP Novosphingobium capsulatum
3.4.21.26 R354K mutant of SC PEP Novosphingobium capsulatum
3.4.21.26 R481K mutant of SC PEP Novosphingobium capsulatum
3.4.21.26 S351P mutant of SC PEP Novosphingobium capsulatum
3.4.21.26 V323I mutant of SC PEP Novosphingobium capsulatum
3.4.21.26 V346I mutant of SC PEP Novosphingobium capsulatum
3.4.21.26 V474I one of the mutations that shows the most significant correlation to improved activity and/or stability under simulated gastric conditions Novosphingobium capsulatum
3.4.21.26 V500I mutant of SC PEP Novosphingobium capsulatum
3.4.21.26 Y669F mutant of SC PEP Novosphingobium capsulatum

General Stability

EC Number General Stability Organism
3.4.21.26 Enhancing the activity and stability of PEPs under gastric conditions (low pH, high pepsin concentration) is a challenge for protein engineers Novosphingobium capsulatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.26 additional information Novosphingobium capsulatum ability to cleave immunotoxic gluten peptides endoproteolytically, attractive oral therapeutic candidates for protecting celiac sprue patients from the toxic effects of dietary gluten ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.26 Novosphingobium capsulatum Q9ZNM8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.26
-
 Novosphingobium capsulatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.26 additional information ability to cleave immunotoxic gluten peptides endoproteolytically, attractive oral therapeutic candidates for protecting celiac sprue patients from the toxic effects of dietary gluten Novosphingobium capsulatum ?
-
 ?
3.4.21.26 succinyl-Ala-Pro-p-nitroanilide + H2O
-
 Novosphingobium capsulatum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.26 prolyl endopeptidase
-
 Novosphingobium capsulatum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.26 4.5
-
 variants with as much as 20% enhanced specific activity at pH 4.5 and 200fold greater resistance to pepsin identified Novosphingobium capsulatum