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Literature summary extracted from

  • Stevens, R.C.; Suzuki, S.M.; Cole, T.B.; Park, S.S.; Richter, R.J.; Furlong, C.E.
    Engineered recombinant human paraoxonase 1 (rHuPON1) purified from Escherichia coli protects against organophosphate poisoning (2008), Proc. Natl. Acad. Sci. USA, 105, 12780-12784.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.2 expressed in Escherichia coli Homo sapiens
3.1.8.1 expressed in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.2 0.957
-
 phenyl acetate recombinant wild type enzyme, in 0.1 M Tris-HCl (pH 8.5), 2 M NaCl, and 2 mM CaCl2 Homo sapiens
3.1.8.1 0.131
-
 chlorpyrifos recombinant wild type enzyme, in 0.1 M Tris-HCl (pH 8.5), 2 M NaCl, and 2 mM CaCl2 Homo sapiens
3.1.8.1 0.868
-
 paraoxon recombinant wild type enzyme, in 0.1 M Tris-HCl (pH 8.5), 2 M NaCl, and 2 mM CaCl2 Homo sapiens
3.1.8.1 1.33
-
 diazoxon recombinant wild type enzyme, in 0.1 M Tris-HCl (pH 8.5), 2 M NaCl, and 2 mM CaCl2 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Homo sapiens P27169
-
-
3.1.8.1 Homo sapiens P27169
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.2 ceramic HA column chromatography, DEAE-Sepharose column chromatography, Sephadex G-25 gel filtration, and tert-butyl Sepharose column chromatography Homo sapiens
3.1.8.1 ceramic HA column chromatography, DEAE-Sepharose column chromatography, Sephadex G-25 gel filtration, and tert-butyl Sepharose column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.2 5.15
-
 recombinant enzyme from crude cell extract Homo sapiens
3.1.1.2 465
-
 recombinant enzyme after 90fold purification Homo sapiens
3.1.8.1 5.15
-
 recombinant enzyme from crude cell extract Homo sapiens
3.1.8.1 465
-
 recombinant enzyme after 90fold purification Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.1.1.2 4°C, purified recombinant enzyme in 20 mM Tris pH 7.7, 1 mM CaCl2, at least one month, remains stable Homo sapiens
3.1.8.1 4°C, purified recombinant enzyme in 20 mM Tris pH 7.7, 1 mM CaCl2, at least one month, remains stable Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 phenyl acetate + H2O
-
 Homo sapiens phenol + acetate
-
 ?
3.1.8.1 chlorpyrifos + H2O
-
 Homo sapiens 3,5,6-trichloro-pyridin-2-ol + diethyl thiophosphate
-
 ?
3.1.8.1 diazoxon + H2O
-
 Homo sapiens ?
-
 ?
3.1.8.1 paraoxon + H2O
-
 Homo sapiens diethylphosphate + 4-nitrophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.2 AREase
-
 Homo sapiens
3.1.8.1 paraoxonase 1
-
 Homo sapiens
3.1.8.1 PON1
-
 Homo sapiens