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Literature summary extracted from

  • Yajun, H.; Wangzhen, G.; Xinlian, S.; Tianzhen, Z.
    Molecular cloning and characterization of a cytosolic glutamine synthetase gene, a fiber strength-associated gene in cotton (2008), Planta, 228, 473-483.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.2 gene GhGS, DNA and amino acid sequence determination and analysis, molecular mapping of the GhGS gene, tissue-specific expression analysis Gossypium hirsutum

Protein Variants

EC Number Protein Variants Comment Organism
6.3.1.2 additional information identification of single nucleotide polymorphisms of the genomic GhGS sequences in 7235 and TM-1 Gossypium hirsutum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.1.2 chloroplast isozyme GS2 Gossypium hirsutum 9507
-
6.3.1.2 cytosol isozyme GS1, the enzyme is fiber associated Gossypium hirsutum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Mg2+
-
 Gossypium hirsutum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.2 ATP + L-glutamate + NH3 Gossypium hirsutum
-
 ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 additional information Gossypium hirsutum GS is involved in the assimilation of ammonia in the plant. GS catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Gossypium hirsutum A8WCU3 gene GhGS; allotetraploid, strains 7235 and TM-1, gene GhGS, isozymes GS1 and GS2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.2 fiber association with Gossypium hirsutum
-
6.3.1.2 leaf
-
 Gossypium hirsutum
-
6.3.1.2 ovum
-
 Gossypium hirsutum
-
6.3.1.2 root
-
 Gossypium hirsutum
-
6.3.1.2 stem
-
 Gossypium hirsutum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 additional information
-
 activity and expression of GS during ovule and fiber development, overview Gossypium hirsutum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-glutamate + NH3
-
 Gossypium hirsutum ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 additional information GS is involved in the assimilation of ammonia in the plant. GS catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine Gossypium hirsutum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 octamer
-
 Gossypium hirsutum

Synonyms

EC Number Synonyms Comment Organism
6.3.1.2 Glutamine synthetase
-
 Gossypium hirsutum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.2 37
-
 assay at Gossypium hirsutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 8
-
 assay at Gossypium hirsutum

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.2 ATP
-
 Gossypium hirsutum