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Literature summary extracted from

  • Kogawa, K.; Kato, N.; Kazuma, K.; Noda, N.; Suzuki, M.
    Purification and characterization of UDP-glucose: anthocyanin 3',5'-O-glucosyltransferase from Clitoria ternatea (2007), Planta, 226, 1501-1509.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.249 diethyldicarbonate 10 mM, 16% activity retains Clitoria ternatea
2.4.1.249 dithioerythritol 10 mM, 83% activity retains Clitoria ternatea
2.4.1.249 iodoacetate 10 mM, 84% activity retains Clitoria ternatea
2.4.1.249 N-ethylmaleimide 10 mM, 16% activity retains Clitoria ternatea
2.4.1.249 p-chloromercuribenzoic acid 10 mM, completely inhibited Clitoria ternatea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.249 0.0389
-
 delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside
-
 Clitoria ternatea
2.4.1.249 0.138
-
 delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside
-
 Clitoria ternatea
2.4.1.249 1.49
-
 UDP-D-glucose apparent, delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as saturated substrate Clitoria ternatea
2.4.1.249 6.18
-
 UDP-D-glucose apparent, delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside as saturated substrate Clitoria ternatea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.249 CaCl2 10 mM, 82% activity retains Clitoria ternatea
2.4.1.249 EDTA 10 mM, 75% activity retains Clitoria ternatea
2.4.1.249 FeCl2 10 mM, 73% activity retains Clitoria ternatea
2.4.1.249 MgCl2 10 mM, 88% activity retains Clitoria ternatea
2.4.1.249 additional information completely inhibited by 10 mM CuCl2 Clitoria ternatea
2.4.1.249 ZnCl2 10 mM, 62% activity retains Clitoria ternatea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.249 48000
-
 gel filtration Clitoria ternatea
2.4.1.249 50800
-
 SDS-PAGE Clitoria ternatea
2.4.1.249 58000
-
 1 * 58000, SDS-PAGE Clitoria ternatea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.249 UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside Clitoria ternatea 3'-O-glucosyltransferase activity, first reaction of UA3'5'GT in the biosynthesis of ternatins, transferring two glucosyl groups in a stepwise manner UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside
-
 ?
2.4.1.249 UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3'-O-beta-D-glucoside Clitoria ternatea 5'-O-glucosyltransferase activity, second reaction of UA3'5'GT in the biosynthesis of ternatins, transferring two glucosyl groups in a stepwise manner UDP + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3',5'-di-O-beta-D-glucoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.249 Clitoria ternatea A4F1Q6 gene An3'5'GT; butterfly pea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.249 from petals, which accumulate polyacylated anthocyanins and ternatins, using ammonium sulfate precipitation, DEAE Toyopearl, Reactive yellow 86, Cellufine HAP, and Mono Q chromatography, UA5'GT: 1007fold purified, UA3'GT: 346 fold purified Clitoria ternatea

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.249 UDP-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside = UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-D-glucoside UDP-glucose:anthocyanin 3',5'-O-glucosyltransferase has both, UA3'GT and UA5'GT activities, highly regioselective to the 3' and 5' OH groups Clitoria ternatea
a
2.4.1.249 UDP-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-D-glucoside = UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3',5'-di-O-beta-D-glucoside UDP-glucose:anthocyanin 3',5'-O-glucosyltransferase has both, UA3'GT and UA5'GT activities, highly regioselective to the 3' and 5' OH groups Clitoria ternatea
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.249 petal
-
 Clitoria ternatea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.249 0.000276
-
 UA5'GT activity, substrate: delphinidin 3,3'-di-O-beta-D-glucoside Clitoria ternatea
2.4.1.249 0.000324
-
 UA3'GT activity, substrate: delphinidin 3-O-beta-D-glucoside Clitoria ternatea
2.4.1.249 0.002
-
 UA3'GT activity, substrate: cyanidin 3-O-(6''-O-malonyl)-beta-D-glucoside Clitoria ternatea
2.4.1.249 0.01
-
 UA5'GT activity, substrate: petunidin 3-O-(6''-O-malonyl)-beta-D-glucoside Clitoria ternatea
2.4.1.249 0.98
-
 UA3'GT activity, substrate: delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside Clitoria ternatea
2.4.1.249 2.29
-
 UA5'GT activity, substrate: delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside Clitoria ternatea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.249 additional information no activity detected with cyanidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-D-glucoside, pelargonidin 3-O-(6''-O-malonyl)-beta-D-glucoside, delphinidin 3,5-di-O-beta-D-glucoside. No activity with delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3'-O-beta-D-glucoside using UDP-D-galactose as sugar donor Clitoria ternatea ?
-
 ?
2.4.1.249 UDP-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
 Clitoria ternatea UDP + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside 3'-O-beta-D-glucoside 24.3% relative activity compared to delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as substrate ?
2.4.1.249 UDP-D-glucose + delphinidin 3,3'-di-O-beta-D-glucoside 3.3% relative activity compared to delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as substrate Clitoria ternatea UDP + delphinidin 3,3',5'-tri-O-beta-D-glucoside
-
 ?
2.4.1.249 UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside 3'-O-glucosyltransferase activity, first reaction of UA3'5'GT in the biosynthesis of ternatins, transferring two glucosyl groups in a stepwise manner Clitoria ternatea UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside
-
 ?
2.4.1.249 UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside 3'-O-glucosyltransferase activity, catalyzed by UA3'5'GT Clitoria ternatea UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside
-
 ?
2.4.1.249 UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3'-O-beta-D-glucoside 5'-O-glucosyltransferase activity, second reaction of UA3'5'GT in the biosynthesis of ternatins, transferring two glucosyl groups in a stepwise manner Clitoria ternatea UDP + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3',5'-di-O-beta-D-glucoside
-
 ?
2.4.1.249 UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3'-O-beta-D-glucoside 5'-O-glucosyltransferase activity, catalyzed by UA3'5'GT Clitoria ternatea UDP + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3',5'-di-O-beta-D-glucoside
-
 ?
2.4.1.249 UDP-D-glucose + delphinidin 3-O-beta-D-glucoside 3.9% relative activity compared to delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as substrate Clitoria ternatea UDP + delphinidin 3,3'-di-O-beta-D-glucoside
-
 ?
2.4.1.249 UDP-D-glucose + petunidin 3-O-(6''-O-malonyl)-beta-D-glucoside
-
 Clitoria ternatea UDP + petunidin 3-O-(6''-O-malonyl)-beta-D-glucoside 5'-O-beta-D-glucoside 123% relative activity compared to delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as substrate ?

Subunits

EC Number Subunits Comment Organism
2.4.1.249 monomer 1 * 58000, SDS-PAGE Clitoria ternatea

Synonyms

EC Number Synonyms Comment Organism
2.4.1.249 UA3'5'GT
-
 Clitoria ternatea
2.4.1.249 UA3'GT
-
 Clitoria ternatea
2.4.1.249 UA5'GT
-
 Clitoria ternatea
2.4.1.249 UDP-glucose:anthocyanin 3',5'-O-glucosyltransferase
-
 Clitoria ternatea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.249 4.4
-
 delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside UDP-glucose as sugar donor Clitoria ternatea
2.4.1.249 4.9
-
 UDP-D-glucose delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside as substrate Clitoria ternatea
2.4.1.249 5.1
-
 UDP-D-glucose delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as substrate Clitoria ternatea
2.4.1.249 5.4
-
 delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside UDP-glucose as sugar donor Clitoria ternatea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.249 7.5 8 pH 7.5 and pH 8.0 in potassium phosphate buffer and Tris-HCl buffer, respectively Clitoria ternatea