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Literature summary extracted from

  • Johansson, M.; Hammargren, J.; Uppsaell, E.; MacKenzie, A.; Knorpp, C.
    The activities of nucleoside diphosphate kinase and adenylate kinase are influenced by their interaction (2008), Plant Sci., 174, 192-199.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.6 additional information adenylate kinase from Arabidopsis thaliana stimulates NDPK activity, whereas NDPK inhibits the activity of adenylate kinase, overview Pisum sativum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.3 expression in Escherichia coli Arabidopsis thaliana
2.7.4.6 expression of His-tagged NDPK in Escherichia coli Pisum sativum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.6 additional information immobiliztaion of the purified recombinant enzyme on N-hydroxysuccinimide HS-activated HP resin Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.6 0.126
-
 ATP recombinant His-tagged NDPK Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.3 mitochondrion most highly expressed isoform AK1 Arabidopsis thaliana 5739
-
2.7.4.6 mitochondrion
-
 Pisum sativum 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.6 Mg2+
-
 Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.3 additional information Arabidopsis thaliana interaction between mitochondrial adenylate kinase and nucleoside diphosphate kinase. Adenylate kinase stimulates nucleoside diphosphate kinase activity, whereas nucleoside diphosphate kinase inhibits adenylate kinase activity. the net effect may be unchanged ADP production albeit with different rates of substrate consumption ?
-
 ?
2.7.4.6 ATP + NDP Pisum sativum
-
 ADP + NTP
-
 r
2.7.4.6 additional information Pisum sativum nucleoside diphosphate kinase is responsible for maintaining the nucleotide balance in the cell, but is also involved in regulatory functions that often are mediated via protein-protein interactions, differentially for adenylate kinase isozymes, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Arabidopsis thaliana O82514 isoform AK1
-
2.7.4.6 Pisum sativum
-
 cv. Oregon sugarpod
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 recombinant His-tagged NDPK from Escherichia coli by nickel affinity chromatography Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 additional information interaction between mitochondrial adenylate kinase and nucleoside diphosphate kinase. Adenylate kinase stimulates nucleoside diphosphate kinase activity, whereas nucleoside diphosphate kinase inhibits adenylate kinase activity. the net effect may be unchanged ADP production albeit with different rates of substrate consumption Arabidopsis thaliana ?
-
 ?
2.7.4.6 ATP + GDP
-
 Pisum sativum ADP + GTP
-
 r
2.7.4.6 ATP + NDP
-
 Pisum sativum ADP + NTP
-
 r
2.7.4.6 additional information nucleoside diphosphate kinase is responsible for maintaining the nucleotide balance in the cell, but is also involved in regulatory functions that often are mediated via protein-protein interactions, differentially for adenylate kinase isozymes, overview Pisum sativum ?
-
 ?
2.7.4.6 additional information protein-protein interaction analysis of adenylate kinases from Arabidopsis thaliana with the immobilized recombinant His-tagged NDPK from Pisum sativum, the interaction influences both enzyme activities, overview Pisum sativum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.3 More interaction between mitochondrial adenylate kinase and nucleoside diphosphate kinase. Adenylate kinase stimulates nucleoside diphosphate kinase activity, whereas nucleoside diphosphate kinase inhibits adenylate kinase activity. The net effect may be unchanged ADP production albeit with different rates of substrate consumption Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.7.4.6 NDPK
-
 Pisum sativum
2.7.4.6 nucleoside diphosphate kinase
-
 Pisum sativum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.6 2085
-
 ATP recombinant His-tagged NDPK Pisum sativum