Literature summary extracted from

Tuttle, J.R.; Idris, A.M.; Brown, J.K.; Haigler, C.H.; Robertson, D.
Geminivirus-mediated gene silencing from Cotton leaf crumple virus is enhanced by low temperature in cotton (2008), Plant Physiol., 148, 41-50.

Application

EC Number	Application	Comment	Organism
6.6.1.1	agriculture	use of the CLCrV silencing vector to study gene function in cotton, via replacement of the CLCrV coat protein gene by up to 500 bp of DNA homologous to the magnesium chelatase subunit I gene ChlI. Temperature can have a major impact on the extent of geminivirus-induced gene silencing	Gossypium hirsutum

Protein Variants

EC Number	Protein Variants	Comment	Organism
6.6.1.1	additional information	gene silencing by use of silencing vector from the geminivirus Cotton leaf crumple virus (CLCrV), via replacement of the CLCrV coat protein gene by up to 500 bp of DNA homologous to the magnesium chelatase subunit I gene ChlI. Cotyledons of cotton cultivar Deltapine 5415 bombarded with the modified viral vectors manifest chlorosis in approximately 70% of inoculated plants after 2 to 3 weeks. Replication of viral DNA is restricted to vascular tissue and the viral vector can transmit to leaves, roots, and the ovule integument from which fibers originate. Plants grown at a 30°C/26°C day/night cycle have a greater than 10fold reduction in viral DNA accumulation compared to plants grown at 22°C/18°C	Gossypium hirsutum

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.6.1.1	Gossypium hirsutum	-	-	-

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Release 2023.1 (January 2023)