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Literature summary extracted from

  • Teranishi, M.; Nakamura, K.; Morioka, H.; Yamamoto, K.; Hidema, J.
    The native cyclobutane pyrimidine dimer photolyase of rice is phosphorylated (2008), Plant Physiol., 146, 1941-1951.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.3 expressed in Escherichia coli, the purified native rice CPD photolyase has significantly higher CPD photorepair activity than the Escherichia coli-expressed CPD photolyase, the structure of the native rice CPD photolyase differs significantly from that of the Escherichia coli-expressed rice CPD photolyase, and the structural modification of the native CPD photolyase leads to higher activity Oryza sativa Japonica Group

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.3 54000
-
 purified native unphosphorylated CPD photolyase bound to CPD-containing DNA conjugated to magnetic beads and then released by blue-light irradiation, SDS-PAGE Oryza sativa Japonica Group
4.1.99.3 55000
-
 unphosphorylated recombinant enzyme, SDS-PAGE Oryza sativa Japonica Group
4.1.99.3 56000
-
 purified native phosphorylated CPD photolyase bound to CPD-containing DNA conjugated to magnetic beads and then released by blue-light irradiation, SDS-PAGE Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.3 additional information Oryza sativa Japonica Group the native rice CPD photolyase is phosphorylated, whereas the Escherichia coli-expressed rice CPD photolyase is not ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.3 Oryza sativa Japonica Group Q6F6A2
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.1.99.3 phosphoprotein the native rice CPD photolyase is phosphorylated, whereas the Escherichia coli-expressed rice CPD photolyase is not Oryza sativa Japonica Group

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.3 ammonium sulfate fractionation, UNO-Q12 column chromatography, and heparin affinity column chromatography, the purified native rice CPD photolyase has significantly higher CPD photorepair activity than the Escherichia coli-expressed CPD photolyase Oryza sativa Japonica Group

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.99.3 leaf
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.3 cyclobutadipyrimidine in DNA
-
 Oryza sativa Japonica Group pyrimidine residues in DNA
-
 ?
4.1.99.3 additional information the native rice CPD photolyase is phosphorylated, whereas the Escherichia coli-expressed rice CPD photolyase is not Oryza sativa Japonica Group ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.99.3 ? x * 56000, SDS-PAGE Oryza sativa Japonica Group

Synonyms

EC Number Synonyms Comment Organism
4.1.99.3 CPD photolyase absorbs blue/UVA light as an energy source to monomerize dimers Oryza sativa Japonica Group
4.1.99.3 cyclobutane pyrimidine dimer photolyase
-
 Oryza sativa Japonica Group

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.3 FAD
-
 Oryza sativa Japonica Group