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Literature summary extracted from

  • Koprivova, A.; North, K.A.; Kopriva, S.
    Complex signaling network in regulation of adenosine 5-phosphosulfate reductase by salt stress in Arabidopsis roots (2008), Plant Physiol., 146, 1408-1420.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.99.2 NaCl APR activity of all three isoforms increases 3fold in roots after 5 h of treatment with 150 mM NaCl Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.9 additional information regulation of APR by NaCl is not affected in mutant plants deficient in the abscisic acid synthesis, but APR is induced in mutant plants deficient in jasmonate, salicylate, ethylene, cytokinin, and auxin signaling, while the enzyme activity remians unaltered, the induction by salt is abolished in mutant plants deficient in gibberellic acid signaling, overview Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.4.9 NaCl all three APR isoforms increased 3fold in roots after 5 h of treatment with 150 mM NaCl, regulation of salt stress by plant hormone signalling, overview Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.99.2 52000
-
 SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.9 adenylyl sulfate + glutathione Arabidopsis thaliana
-
 AMP + sulfite + glutathione disulfide
-
 ?
1.8.4.9 additional information Arabidopsis thaliana APR is the key enzyme of sulfate assimilation, extensive posttranscriptional regulation of plant APR, e.g. by salt stress, sulfate assimilation pathway is controlled by a complex network of multiple signals on different regulatory levels, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.9 Arabidopsis thaliana
-
 Columbia-0 ecotype, three APR isozymes
-
1.8.99.2 Arabidopsis thaliana
-
 ecotype Columbia-0
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.9 root
-
 Arabidopsis thaliana
-
1.8.99.2 leaf
-
 Arabidopsis thaliana
-
1.8.99.2 root
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.9 adenylyl sulfate + glutathione
-
 Arabidopsis thaliana AMP + sulfite + glutathione disulfide
-
 ?
1.8.4.9 additional information APR is the key enzyme of sulfate assimilation, extensive posttranscriptional regulation of plant APR, e.g. by salt stress, sulfate assimilation pathway is controlled by a complex network of multiple signals on different regulatory levels, overview Arabidopsis thaliana ?
-
 ?
1.8.99.2 adenylyl sulfate + dithioerythritol
-
 Arabidopsis thaliana AMP + sulfite + oxidized dithioerythritol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.99.2 ? x * 52000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.8.4.9 adenosine 5-phosphosulfate reductase
-
 Arabidopsis thaliana
1.8.99.2 adenosine 5'-phosphosulfate reductase
-
 Arabidopsis thaliana
1.8.99.2 APR1 isoform Arabidopsis thaliana
1.8.99.2 APR2 isoform Arabidopsis thaliana
1.8.99.2 APR3 isoform Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.9 glutathione
-
 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
1.8.99.2 Arabidopsis thaliana APR mRNA levels of all three isoforms increase 3fold in roots after 5 h of treatment with 150 mM NaCl. In mutants deficient in cytokinin and auxin, jasmonate, salicylate, or ethylene signaling APR mRNA levels are increased upon salt exposure similar to wild type plants, however, APR enzyme activity is not affected by salt in these plants. Signaling via gibberellic acid is essential for the increase in APR mRNA by salt treatment. Addition of 0.1 mM salicylate to the nutrient solution leads to an increase of mRNA levels of all three APR isoforms. 0.2 mM 1-aminocyclopropane carboxylic acid and 0.045 mM jasmonate increase accumulation of APR1 and APR3 transcripts, but do not affect the APR2 isoform. up