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Literature summary extracted from

  • Endler, A.; Martens, S.; Wellmann, F.; Matern, U.
    Unusually divergent 4-coumarate:CoA-ligases from Ruta graveolens L (2008), Plant Mol. Biol., 67, 335-346.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 expressed in Saccharomyces cerevisiae Ruta graveolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.0031
-
 4-coumarate isozyme 4CL2, at pH 7.8 and 32°C Ruta graveolens
6.2.1.12 0.0055
-
 4-coumarate isozyme 4CL1, pH 7.8, 32°C Ruta graveolens
6.2.1.12 0.037
-
 caffeate isozyme 4CL2, at pH 7.8 and 32°C Ruta graveolens
6.2.1.12 0.037
-
 cinnamate isozyme 4CL2, at pH 7.8 and 32°C Ruta graveolens
6.2.1.12 0.0484
-
 caffeate isozyme 4CL1, pH 7.8, 32°C Ruta graveolens
6.2.1.12 0.059
-
 cinnamate isozyme 4CL1, pH 7.8, 32°C Ruta graveolens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.12 62380
-
 calculated from amino acid sequence, isozyme 4CL1 Ruta graveolens
6.2.1.12 62384
-
 x * 62384, calculated from amino acid sequence, isozyme 4CL1 Ruta graveolens

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Ruta graveolens B3TJI4 isozyme 4CL1
-
6.2.1.12 Ruta graveolens B3TJI5 isozyme 4CL2
-
6.2.1.12 Ruta graveolens R-20 B3TJI4 isozyme 4CL1
-
6.2.1.12 Ruta graveolens R-20 B3TJI5 isozyme 4CL2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 Sephadex G-25 gel filtration Ruta graveolens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 flower
-
 Ruta graveolens
-
6.2.1.12 flower isozyme 4CL1 is mostly expressed in the flower Ruta graveolens
-
6.2.1.12 leaf
-
 Ruta graveolens
-
6.2.1.12 root negligible expression in the root Ruta graveolens
-
6.2.1.12 shoot
-
 Ruta graveolens
-
6.2.1.12 shoot isozyme 4CL2 is expressed mostly in the shoot Ruta graveolens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA 100% activity Ruta graveolens AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA 100% activity Ruta graveolens R-20 AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA 48% activity compared to 4-coumarate Ruta graveolens AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA 86% activity compared to 4-coumarate Ruta graveolens AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA 86% activity compared to 4-coumarate Ruta graveolens R-20 AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA 48% activity compared to 4-coumarate Ruta graveolens R-20 AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA 5.0% activity compared to 4-coumarate Ruta graveolens AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA 54% activity compared to 4-coumarate Ruta graveolens AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA 54% activity compared to 4-coumarate Ruta graveolens R-20 AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA 5.0% activity compared to 4-coumarate Ruta graveolens R-20 AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA 2.5% activity compared to 4-coumarate Ruta graveolens AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA 50.5% activity compared to 4-coumarate Ruta graveolens AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA 2.5% activity compared to 4-coumarate Ruta graveolens R-20 AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA 50.5% activity compared to 4-coumarate Ruta graveolens R-20 AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 additional information the enzyme does not activate sinapate, 2,4-dihydroxybenzoic acid, 4-hydroxybenzoic acid, acetylsalicylic acid, anthranilic acid, and (N-methyl)anthranilic acid Ruta graveolens ?
-
 ?
6.2.1.12 additional information the enzyme does not activate sinapate, 2,4-dihydroxybenzoic acid, 4-hydroxybenzoic acid, acetylsalicylic acid, anthranilic acid, and (N-methyl)anthranilic acid Ruta graveolens R-20 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.12 ? x * 62384, calculated from amino acid sequence, isozyme 4CL1 Ruta graveolens

Synonyms

EC Number Synonyms Comment Organism
6.2.1.12 4-coumarate:CoA-ligase
-
 Ruta graveolens
6.2.1.12 4CL
-
 Ruta graveolens
6.2.1.12 4CL1 isozyme Ruta graveolens
6.2.1.12 4CL2 isozyme Ruta graveolens

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.12 ATP
-
 Ruta graveolens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.2.1.12 Ruta graveolens calculated from amino acid sequence, isozyme 4CL1
-
 5.8