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Literature summary extracted from

  • Fortunati, A.; Barta, C.; Brilli, F.; Centritto, M.; Zimmer, I.; Schnitzler, J.P.; Loreto, F.
    Isoprene emission is not temperature-dependent during and after severe drought-stress: a physiological and biochemical analysis (2008), Plant J., 55, 687-697.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.3.27 isoprene biosynthesis by ISPS is relatively unaffected by drought Populus nigra

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.27 additional information When photosynthesis is completely inhibited by a 35-day drought stress, the isoprene emission of plants grown at 25°C and 35°C is strongly inhibited but still measurable, averaging 7.5 nmol/qm/sec. After a 3-day re-watering phase, the isoprene emission rate recovers in plants grown at both 25°C and 35°C. Populus nigra

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.27 chloroplast
-
 Populus nigra 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.27 Mg2+ included in assay medium Populus nigra

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate Populus nigra
-
 isoprene + diphosphate isoprene is an important molecule for ameliorating abiotic stresses ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.27 Populus nigra A0PFK2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.27 leaf
-
 Populus nigra
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.27 additional information
-
 A much higher PnISPS activity sustains high rates of isoprene emission in plants grow at 35°C before drought stress. Large reductions of PnISPS activity have no relevant effect on isoprene emission in conditions of mild drought stress. The PnISPS activities of leaves grown at 35°C do not recover the original rates once drought stress is relieved. Populus nigra
4.2.3.27 additional information
-
 ISPS activity decreases before isoprene emission during drought development, indicating a tighter control of the emission at a transcriptional or post-transcriptional level under moderate drought stress, at 25°C and 35°C. Populus nigra
4.2.3.27 additional information
-
 PnISPS activity is generally related to isoprene emission rates along the drought treatment Populus nigra
4.2.3.27 additional information
-
 Prior to the imposition of drought stress, plants grown at 35°C show much higher PnISPS activities than plants grown at 25°C. Drought stress negatively influences PnISPS activity, especially in plants grow at 35°C. In these plants, PnISPS activity starts to decrease when drought stress is mild and only partially recovered after re-watering. In plants grown at 25°C, the inhibition of PnISPS activity occurred more slowly and only became evident when the stress is severe. At 25°C, PnISPS activity recovers rapidly and completely upon re-watering. Populus nigra

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate
-
 Populus nigra isoprene + diphosphate isoprene is an important molecule for ameliorating abiotic stresses ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.27 isoprene synthase
-
 Populus nigra
4.2.3.27 ISPS
-
 Populus nigra
4.2.3.27 PnISPS
-
 Populus nigra

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.27 30
-
 assay at Populus nigra