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Literature summary extracted from

  • Kamada-Nobusada, T.; Hayashi, M.; Fukazawa, M.; Sakakibara, H.; Nishimura, M.
    A putative peroxisomal polyamine oxidase, AtPAO4, is involved in polyamine catabolism in Arabidopsis thaliana (2008), Plant Cell Physiol., 49, 1272-1282.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.16 expression in Escherichia coli as a His-tagged protein Arabidopsis thaliana
1.5.3.16 expression of His-tagged AtPAO4 in Escherichia coli strain BL21(DE3), transient expression of isozyme AtPAO4 in Arabidopsis thaliana root cell peroxisomes as monomeric red fluorescent protein fusion protein Arabidopsis thaliana
1.5.3.17 transient expression of isozyme AtPAO3 in Arabidopsis thaliana root cell peroxisomes as monomeric red fluorescent protein fusion protein Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.5.3.16 additional information construction of a knock-out mutant that has a T-DNA insertion within the ninth exon of the AtPAO4 gene, and of a knock-down mutant Atpao4i with reduced expression by RNAi under control of the CMV 35S promoter. AtPAO4 deficiency induces alterations in the expression of genes related to the drought stress response and flavonoid biosynthesis Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.16 0.23
-
spermine 25°C, pH 8 Arabidopsis thaliana
1.5.3.16 0.23
-
spermine recombinant His-tagged isozyme AtPAO4 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.3.16 peroxisome
-
Arabidopsis thaliana 5777
-
1.5.3.16 peroxisome AtPAO4 is the major isoform in root peroxisomes Arabidopsis thaliana 5777
-
1.5.3.17 peroxisome
-
Arabidopsis thaliana 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.16 55000
-
x * 55000, SDS-PAGE Arabidopsis thaliana
1.5.3.16 55000
-
x * 55000, recombinant His-tagged AtPAO4, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.16 additional information Arabidopsis thaliana AtPAO4 deficiency induces alterations in the expression of genes related to the drought stress response and flavonoid biosynthesis ?
-
?
1.5.3.16 additional information Arabidopsis thaliana peroxisomal polyamine oxidase AtPAO4 is involved in the catabolism of polyamines in leaves, overview ?
-
?
1.5.3.16 spermine + O2 + H2O Arabidopsis thaliana
-
spermidine + 3-aminopropanal + H2O2
-
?
1.5.3.17 spermine + O2 + H2O Arabidopsis thaliana
-
spermidine + 3-aminopropanal + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.16 Arabidopsis thaliana Q8H191
-
-
1.5.3.16 Arabidopsis thaliana Q8H191 isozyme AtPAO4
-
1.5.3.17 Arabidopsis thaliana Q9LYT1 isozyme AtPAO3
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.5.3.16 flavoprotein
-
Arabidopsis thaliana
1.5.3.17 flavoprotein
-
Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.16
-
Arabidopsis thaliana
1.5.3.16 recombinant His-tagged AtPAO4 from Escherichia coli strain BL21(DE3) Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.3.16 cotyledon greening Arabidopsis thaliana
-
1.5.3.16 flower
-
Arabidopsis thaliana
-
1.5.3.16 leaf
-
Arabidopsis thaliana
-
1.5.3.16 additional information isozyme AtPAO4 transcript is abundantly expressed in roots, flowers and greening cotyledons, but more poorly expressed in other tissues Arabidopsis thaliana
-
1.5.3.16 root
-
Arabidopsis thaliana
-
1.5.3.16 root AtPAO4 is the major isoform in root peroxisomes Arabidopsis thaliana
-
1.5.3.16 seedling
-
Arabidopsis thaliana
-
1.5.3.16 silique
-
Arabidopsis thaliana
-
1.5.3.16 stem
-
Arabidopsis thaliana
-
1.5.3.17 cotyledon greening Arabidopsis thaliana
-
1.5.3.17 flower
-
Arabidopsis thaliana
-
1.5.3.17 leaf
-
Arabidopsis thaliana
-
1.5.3.17 additional information isozyme AtPAO3 is abundantly expressed in all tissues Arabidopsis thaliana
-
1.5.3.17 root
-
Arabidopsis thaliana
-
1.5.3.17 seedling
-
Arabidopsis thaliana
-
1.5.3.17 silique
-
Arabidopsis thaliana
-
1.5.3.17 stem
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.16 additional information AtPAO4 deficiency induces alterations in the expression of genes related to the drought stress response and flavonoid biosynthesis Arabidopsis thaliana ?
-
?
1.5.3.16 additional information no activity with putrescine, spermidine, N1-acetylspermine Arabidopsis thaliana ?
-
?
1.5.3.16 additional information peroxisomal polyamine oxidase AtPAO4 is involved in the catabolism of polyamines in leaves, overview Arabidopsis thaliana ?
-
?
1.5.3.16 spermine + O2 + H2O
-
Arabidopsis thaliana spermidine + 3-aminopropanal + H2O2
-
?
1.5.3.16 spermine + O2 + H2O AtPAO4 specifically catalyzes the conversion of spermine to spermidine under the assay conditions Arabidopsis thaliana spermidine + 3-aminopropanal + H2O2
-
?
1.5.3.17 spermine + O2 + H2O
-
Arabidopsis thaliana spermidine + 3-aminopropanal + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.5.3.16 ? x * 55000, SDS-PAGE Arabidopsis thaliana
1.5.3.16 ? x * 55000, recombinant His-tagged AtPAO4, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.5.3.16 AtPAO4
-
Arabidopsis thaliana
1.5.3.17 AtPAO3
-
Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.16 1.4
-
spermine 25°C, pH 8 Arabidopsis thaliana
1.5.3.16 1.4
-
spermine recombinant His-tagged isozyme AtPAO4 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.16 7.5 8.5
-
Arabidopsis thaliana
1.5.3.16 7.5 8.5 recombinant His-tagged isozyme AtPAO4 with substrate spermine Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.16 FAD
-
Arabidopsis thaliana
1.5.3.17 FAD
-
Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.5.3.16 malfunction AtPAO4 deficiency induces alterations in the expression of genes related to the drought stress response and flavonoid biosynthesis Arabidopsis thaliana