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Literature summary extracted from

  • Yamada, T.; Matsuda, F.; Kasai, K.; Fukuoka, S.; Kitamura, K.; Tozawa, Y.; Miyagawa, H.; Wakasa, K.
    Mutation of a rice gene encoding a phenylalanine biosynthetic enzyme results in accumulation of phenylalanine and tryptophan (2008), Plant Cell, 20, 1316-1329.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.51
-
 Oryza sativa
4.2.1.51 expressed in Escherichia coli, mtr1-D mutant gene, callus transformation by Agrobacterium tumefaciens Oryza sativa
4.2.1.91
-
 Oryza sativa

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.51 S298I mutant Mtr1 Oryza sativa
4.2.1.91 S298I mutant Mtr1 Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.51 L-phenylalanine feedback regulation wild-type, mutant enzyme MTR1 (PDT S298I) shows a reduced feedback sensitivity, resulting in phenylalanine accumulation; inhibits activity of wild-type enzyme, mutant enzyme shows reduced feedback sensitivity, accumulation of L-phenylalanine Oryza sativa
4.2.1.91 L-phenylalanine
-
 Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.51 0.12
-
 L-arogenate wild-type, rice PDT is a bifunctional enzyme Oryza sativa
4.2.1.51 0.17
-
 L-arogenate mutant S298I, rice PDT is a bifunctional enzyme Oryza sativa
4.2.1.51 2.29
-
 phenylpyruvate wild-type Oryza sativa
4.2.1.51 2.29
-
 prephenate wild-type, rice PDT is a bifunctional enzyme Oryza sativa
4.2.1.51 4.88
-
 phenylpyruvate Mtr1 mutant Oryza sativa
4.2.1.51 4.88
-
 prephenate mutant S298I, rice PDT is a bifunctional enzyme Oryza sativa
4.2.1.91 0.12
-
 arogenate wild-type, rice ADT is a bifunctional enzyme Oryza sativa
4.2.1.91 0.17
-
 arogenate mutant S298I, rice ADT is a bifunctional enzyme Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.51 chloroplast
-
 Oryza sativa 9507
-
4.2.1.51 chloroplast targeting sequence, in vitro import assay Oryza sativa 9507
-
4.2.1.91 chloroplast
-
 Oryza sativa 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.51 35000
-
 processed to a product of 35000 Dalton during incubation with pea chloroplasts, protected from degradation by thermolysin, organellar targeting sequence Oryza sativa
4.2.1.51 40000
-
 wild-type, SDS-PAGE Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.51 Oryza sativa A8CF65 Oryza sativa subsp. japonica (Rice)
-
4.2.1.51 Oryza sativa Q6Z3Y3 wild-type and Mtr1 mutant
-
4.2.1.91 Oryza sativa A8CF65 Oryza sativa subsp. japonica (Rice)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.51 gel filtration, SDS-PAGE Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.51 callus wild-type, and transformed callus lines expressing mtr1-D mutation, experimental system for feeding experiments with external L-phenylalanine Oryza sativa
-
4.2.1.51 seedling feeding experiments with external L-phenylalanine, accumulation of L-trytophan Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.51 additional information
-
 Mtr1 mutant of rice indicates accumulation of L-phenylalanine, L-trytophan, and of several phenylpropanoids, suggesting a link between the synthesis of L-phenylalanine and L-trytophan, mtr1-D mutant gene indicates point mutation in the putative allosteric regulatory region, wild-type enzyme is feedback regulated by L-phenylalanine, Mtr1 mutant has reduced feedback activity and accumulates L-phenylalanine, critical for regulating the size of the pool of L-phenylalanine in plant cells Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.51 L-arogenate
-
 Oryza sativa L-phenylalanine + CO2 + H2O
-
 ?
4.2.1.51 prephenate bifunctional enzyme, PDT activity with prephenate and ADT activity with arogenate Oryza sativa phenylpyruvate + H2O + CO2
-
 ?
4.2.1.51 prephenate bifunctional enzyme, prephenate dehydratase-arogenate dehydratase, preference for arogenate as substrate indicates primary function as an arogenate dehydratase (ADT) Oryza sativa phenylpyruvate + H2O + CO2
-
 ?
4.2.1.91 L-arogenate bifunctional enzyme, PDT activity with prephenate and ADT activity with arogenate Oryza sativa L-phenylalanine + H2O + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.51 PDT bifunctional enzyme, PDT activity with prephenate and ADT activity with arogenate Oryza sativa
4.2.1.51 PDT protein
-
 Oryza sativa
4.2.1.51 prephenate dehydratase bifunctional enzyme, PDT activity with prephenate and ADT activity with arogenate Oryza sativa
4.2.1.91 AdT bifunctional enzyme, PDT activity with prephenate and ADT activity with arogenate Oryza sativa
4.2.1.91 arogenate dehydratase bifunctional enzyme, PDT activity with prephenate and ADT activity with arogenate Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.51 30
-
 spectrophotometric activity assay at Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.51 8.2
-
 spectrophotometric activity assay at Oryza sativa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.51 7.36
-
 L-phenylalanine wild-type, rice PDT is a bifunctional enzyme Oryza sativa
4.2.1.51 61.5
-
 L-phenylalanine mutant enzyme S298I, rice PDT is a bifunctional enzyme Oryza sativa
4.2.1.91 7.36
-
 L-phenylalanine wild-type, rice ADT is a bifunctional enzyme Oryza sativa
4.2.1.91 61.5
-
 L-phenylalanine mutant enzyme S298I, rice ADT is a bifunctional enzyme Oryza sativa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.51 additional information
-
 L-phenylalanine more than 0.5 Oryza sativa additional information
4.2.1.51 0.016
-
 wild-type, rice PDT is a bifunctional enzyme Oryza sativa L-phenylalanine
4.2.1.91 additional information
-
 L-phenylalanine more than 0.5 Oryza sativa additional information
4.2.1.91 0.016
-
 wild-type, rice ADT is a bifunctional enzyme Oryza sativa L-phenylalanine