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Literature summary extracted from

  • Schildhauer, J.; Wiedemuth, K.; Humbeck, K.
    Supply of nitrogen can reverse senescence processes and affect expression of genes coding for plastidic glutamine synthetase and lysine-ketoglutarate reductase/saccharopine dehydrogenase (2008), Plant Biol., 10 Suppl 1, 76-84.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.2 quantitative RT-PCR expression analysis Hordeum vulgare
6.3.1.2 quantitative RT-PCR expression analysis Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.1.2 chloroplast
-
 Arabidopsis thaliana 9507
-
6.3.1.2 chloroplast isozyme GS2 Hordeum vulgare 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Mg2+
-
 Hordeum vulgare
6.3.1.2 Mg2+
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.2 ATP + L-glutamate + NH3 Hordeum vulgare senescence-specific downregulation of plastidic glutamine synthetase isozyme GS2, this is retarded by fertilisation of plants with nitrate or ammonium, but not urea, at the onset of leaf senescence, GS2 downregulation preceeds upregulation of lysine-ketoglutarate reductase and saccharopine dehydrogenase, overview ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 ATP + L-glutamate + NH3 Arabidopsis thaliana senescence-specific downregulation of plastidic glutamine synthetase, this is retarded by fertilisation of plants with nitrate or ammonium, but not urea, at the onset of leaf senescence, overview ADP + phosphate + L-glutamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.9 Arabidopsis thaliana
-
-
-
1.5.1.9 Hordeum vulgare
-
 cultivar Steffi
-
6.3.1.2 Arabidopsis thaliana Q43127 cv. Columbia
-
6.3.1.2 Hordeum vulgare P13564 cv. Steffi, isozyme GS2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.9 leaf
-
 Hordeum vulgare
-
1.5.1.9 leaf
-
 Arabidopsis thaliana
-
6.3.1.2 leaf flag and foliar leaves, isozyme GS2 expression levels in response to nitrate and ammonium levels, overview Arabidopsis thaliana
-
6.3.1.2 leaf isozyme GS2 expression levels in response to nitrate and ammonium levels, overview Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
 Hordeum vulgare L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 ?
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
 Arabidopsis thaliana L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 ?
6.3.1.2 ATP + L-glutamate + NH3
-
 Hordeum vulgare ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 ATP + L-glutamate + NH3
-
 Arabidopsis thaliana ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 ATP + L-glutamate + NH3 senescence-specific downregulation of plastidic glutamine synthetase isozyme GS2, this is retarded by fertilisation of plants with nitrate or ammonium, but not urea, at the onset of leaf senescence, GS2 downregulation preceeds upregulation of lysine-ketoglutarate reductase and saccharopine dehydrogenase, overview Hordeum vulgare ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 ATP + L-glutamate + NH3 senescence-specific downregulation of plastidic glutamine synthetase, this is retarded by fertilisation of plants with nitrate or ammonium, but not urea, at the onset of leaf senescence, overview Arabidopsis thaliana ADP + phosphate + L-glutamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.9 saccharopine dehydrogenase
-
 Hordeum vulgare
1.5.1.9 saccharopine dehydrogenase
-
 Arabidopsis thaliana
1.5.1.9 SDH
-
 Hordeum vulgare
1.5.1.9 SDH
-
 Arabidopsis thaliana
6.3.1.2 Glutamine synthetase
-
 Hordeum vulgare
6.3.1.2 Glutamine synthetase
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.9 NAD+
-
 Hordeum vulgare
1.5.1.9 NAD+
-
 Arabidopsis thaliana
6.3.1.2 ATP
-
 Hordeum vulgare
6.3.1.2 ATP
-
 Arabidopsis thaliana