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Literature summary extracted from

  • Kaida, R.; Hayashi, T.; Kaneko, T.S.
    Purple acid phosphatase in the walls of tobacco cells (2008), Phytochemistry, 69, 2546-2551.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 brefeldin A blocks secretion during wall regeneration Nicotiana tabacum
3.1.3.2 molybdate
-
 Nicotiana tabacum
3.1.3.2 NaF
-
 Nicotiana tabacum
3.1.3.2 vanadate
-
 Nicotiana tabacum
3.1.3.2 ZnCl2
-
 Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.02
-
 DADE(pY)LIPQQG pH 5.8, 30°C Nicotiana tabacum
3.1.3.2 0.03
-
 END(pY)INASL pH 5.8, 30°C Nicotiana tabacum
3.1.3.2 0.22
-
 RRA(pT)VA pH 5.8, 30°C Nicotiana tabacum
3.1.3.2 0.33
-
 p-nitrophenyl phosphate pH 5.8, 30°C Nicotiana tabacum
3.1.3.2 0.4
-
 ATP pH 5.8, 30°C Nicotiana tabacum
3.1.3.2 0.4
-
 phosphoserine pH 5.8, 30°C Nicotiana tabacum
3.1.3.2 0.41
-
 phosphotyrosine pH 5.8, 30°C Nicotiana tabacum
3.1.3.2 1.45
-
 IDP pH 5.8, 30°C Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.2 cell wall composed as a homotetramer Nicotiana tabacum 5618
-
3.1.3.2 cytoplasm composed as a homodimer Nicotiana tabacum 5737
-
3.1.3.2 additional information double-immunofluorescence labeling results show that the enzyme might be translocated through the Golgi apparatus to the walls at the interphase and to the cell plate during cytokinesis Nicotiana tabacum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Fe2+ enzyme contains Nicotiana tabacum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 60000
-
 2 * 60000, SDS-PAGE in presence of dithiothreitol, in cytoplasm Nicotiana tabacum
3.1.3.2 60000
-
 4 * 60000, SDS-PAGE in presence of dithiothreitol, in the walls Nicotiana tabacum
3.1.3.2 220000
-
 tetramer, SDS-PAGE under non-reducing conditions Nicotiana tabacum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Nicotiana tabacum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 acid phosphatase is eluted from a hydroxyapatite column. First and second butyl-Toyopearl columns are subjected to gel filtration on Superdex 200. The enzyme is purified 213fold Nicotiana tabacum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 494
-
 after final purification step Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Nicotiana tabacum 4-nitrophenol + phosphate
-
 ?
3.1.3.2 ADP + H2O
-
 Nicotiana tabacum AMP + phosphate
-
 ?
3.1.3.2 ATP + H2O
-
 Nicotiana tabacum ?
-
 ?
3.1.3.2 DADE(pY)LIPQQG + H2O tyrosine phosphopeptide Nicotiana tabacum DADEYLIPQQG + phosphate
-
 ?
3.1.3.2 END(pY)INASL + H2O tyrosine phosphopeptide Nicotiana tabacum ENDYINASL + phosphate
-
 ?
3.1.3.2 IDP + H2O
-
 Nicotiana tabacum IMP + phosphate
-
 ?
3.1.3.2 phosphoserine + H2O
-
 Nicotiana tabacum serine + phosphate
-
 ?
3.1.3.2 phosphotyrosine + H2O
-
 Nicotiana tabacum tyrosine + phosphate
-
 ?
3.1.3.2 RRA(pT)VA + H2O serine/threonine phosphopeptide Nicotiana tabacum RRATVA + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 homodimer 2 * 60000, SDS-PAGE in presence of dithiothreitol, in cytoplasm Nicotiana tabacum
3.1.3.2 homotetramer 4 * 60000, SDS-PAGE in presence of dithiothreitol, in the walls Nicotiana tabacum

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 purple acid phosphatase
-
 Nicotiana tabacum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 30
-
 assay at Nicotiana tabacum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.2 2
-
 RRA(pT)VA pH 5.8, 30°C Nicotiana tabacum
3.1.3.2 26.7
-
 DADE(pY)LIPQQG pH 5.8, 30°C Nicotiana tabacum
3.1.3.2 26.8
-
 phosphoserine pH 5.8, 30°C Nicotiana tabacum
3.1.3.2 32.8
-
 END(pY)INASL pH 5.8, 30°C Nicotiana tabacum
3.1.3.2 60.4
-
 IDP pH 5.8, 30°C Nicotiana tabacum
3.1.3.2 62.4
-
 phosphotyrosine pH 5.8, 30°C Nicotiana tabacum
3.1.3.2 125
-
 p-nitrophenyl phosphate pH 5.8, 30°C Nicotiana tabacum
3.1.3.2 133.2
-
 ATP pH 5.8, 30°C Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.8
-
 assay at Nicotiana tabacum