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Literature summary extracted from

  • Silber, M.V.; Meimberg, H.; Ebel, J.
    Identification of a 4-coumarate:CoA ligase gene family in the moss, Physcomitrella patens (2008), Phytochemistry, 69, 2449-2456.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 expressed in Escherichia coli BL21(DE3) cells Physcomitrium patens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.004
-
 4-coumarate recombinant isozyme 4CL1 Physcomitrium patens
6.2.1.12 0.016
-
 4-coumarate recombinant isozyme 4CL2 Physcomitrium patens
6.2.1.12 0.025
-
 caffeate recombinant isozyme 4CL1 Physcomitrium patens
6.2.1.12 0.029
-
 4-coumarate recombinant isozyme 4CL3 Physcomitrium patens
6.2.1.12 0.045
-
 caffeate recombinant isozyme 4CL2 Physcomitrium patens
6.2.1.12 0.05
-
 cinnamate recombinant isozyme 4CL1 Physcomitrium patens
6.2.1.12 0.064
-
 cinnamate recombinant isozyme 4CL3 Physcomitrium patens
6.2.1.12 0.066
-
 cinnamate recombinant isozyme 4CL2 Physcomitrium patens
6.2.1.12 0.073
-
 ferulate recombinant isozyme 4CL2 Physcomitrium patens
6.2.1.12 0.187
-
 ferulate recombinant isozyme 4CL1 Physcomitrium patens
6.2.1.12 0.725
-
 caffeate recombinant isozyme 4CL3 Physcomitrium patens
6.2.1.12 0.8
-
 ferulate recombinant isozyme 4CL3 Physcomitrium patens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.12 64000
-
 SDS-PAGE, isozyme 4CL3 Physcomitrium patens
6.2.1.12 65000
-
 SDS-PAGE, isozyme 4CL1 Physcomitrium patens
6.2.1.12 65000
-
 SDS-PAGE, isozyme 4CL2 Physcomitrium patens

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Physcomitrium patens B7SB99 isozyme 4CL1; subspecies Physcomitrella patens patens
-
6.2.1.12 Physcomitrium patens B7SBA0 isozyme 4CL2; subspecies Physcomitrella patens patens
-
6.2.1.12 Physcomitrium patens B7SBA1 isozyme 4CL3; subspecies Physcomitrella patens patens
-
6.2.1.12 Physcomitrium patens B7SBA2 isozyme 4CL4; subspecies Physcomitrella patens patens
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 Ni-NTA column chromatography Physcomitrium patens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 gametophore
-
 Physcomitrium patens
-
6.2.1.12 protonema
-
 Physcomitrium patens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA highest catalytic efficiency towards 4-coumarate Physcomitrium patens AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Physcomitrium patens AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA
-
 Physcomitrium patens AMP + diphosphate + cinnamoyl-CoA
-
 ir
6.2.1.12 ATP + ferulate + CoA
-
 Physcomitrium patens AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 additional information sinapate is not accepted as a substrate Physcomitrium patens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.12 ? x * 65000, isozyme 4CL1, SDS-PAGE Physcomitrium patens

Synonyms

EC Number Synonyms Comment Organism
6.2.1.12 4-coumarate:CoA ligase
-
 Physcomitrium patens
6.2.1.12 4CL1 isozyme Physcomitrium patens
6.2.1.12 4CL2 isozyme Physcomitrium patens
6.2.1.12 4CL3 isozyme Physcomitrium patens
6.2.1.12 4CL4 isozyme Physcomitrium patens

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.12 ATP
-
 Physcomitrium patens