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Literature summary extracted from

  • Mikami, L.R.; Wieseler, S.; Souza, R.L.; Schopfer, L.M.; Nachon, F.; Lockridge, O.; Chautard-Freire-Maia, E.A.
    Five new naturally occurring mutations of the BCHE gene and frequencies of 12 butyrylcholinesterase alleles in a Brazilian population (2008), Pharmacogenet. Genomics, 18, 213-218.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.8 gene BCHE located at chromosome 3q26.1-q26.2, DNA and amino acid sequence determination and analysis, genotyping in Brasilian population and polymorpshisms, expression of mutants in HEK-293T cells and CHO cells, overview Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.8 A184V naturally occuring mutation in exon 2 Homo sapiens
3.1.1.8 A539T naturally occuring mutation in exon 4 Homo sapiens
3.1.1.8 D70G naturally occuring mutation in exon 2 Homo sapiens
3.1.1.8 E255D naturally occuring mutation in exon 2 Homo sapiens
3.1.1.8 G115D naturally occuring mutation in exon 2 Homo sapiens
3.1.1.8 G333C naturally occuring mutation, the mutant shows about 80% decreases enzyme activity compared to the wild-type enzyme Homo sapiens
3.1.1.8 G390V naturally occuring mutation in exon 2 Homo sapiens
3.1.1.8 K12R naturally occuring mutation in exon 2 Homo sapiens
3.1.1.8 additional information genotyping in Southern Brasilian population and polymorpshisms of the BCHE alleles, overview Homo sapiens
3.1.1.8 R470W naturally occuring mutation in exon 2 Homo sapiens
3.1.1.8 T234M naturally occuring mutation in exon 2 Homo sapiens
3.1.1.8 V294M naturally occuring mutation in exon 2 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.8 0.0118
-
 butyrylthiocholine pH 7.0, 25°C, mutant V294M Homo sapiens
3.1.1.8 0.0169
-
 butyrylthiocholine pH 7.0, 25°C, mutant R470W Homo sapiens
3.1.1.8 0.0273
-
 butyrylthiocholine pH 7.0, 25°C, wild-type enzyme Homo sapiens
3.1.1.8 0.0316
-
 butyrylthiocholine pH 7.0, 25°C, mutant K12R Homo sapiens
3.1.1.8 0.0357
-
 butyrylthiocholine pH 7.0, 25°C, mutant G333C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Homo sapiens
-
 Brasilian population, gene BCHE
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 butyrylthiocholine + H2O detection by 5,5-dithiobis(2-nitrobenzoic acid) Homo sapiens butyrate + thiocholine
-
 ?
3.1.1.8 propionylthiocholine + H2O detection by 5,5-dithiobis(2-nitrobenzoic acid) Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.8 BChE
-
 Homo sapiens
3.1.1.8 butyrylcholinesterase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.8 25
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.8 98
-
 butyrylthiocholine pH 7.0, 25°C, mutant G333C Homo sapiens
3.1.1.8 338
-
 butyrylthiocholine pH 7.0, 25°C, mutant V294M Homo sapiens
3.1.1.8 490
-
 butyrylthiocholine pH 7.0, 25°C, mutant R470W Homo sapiens
3.1.1.8 500
-
 butyrylthiocholine pH 7.0, 25°C, wild-type enzyme Homo sapiens
3.1.1.8 585
-
 butyrylthiocholine pH 7.0, 25°C, mutant K12R Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.8 7
-
 assay at Homo sapiens