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Literature summary extracted from

  • Kammoun, M.; Gargouri, Y.; Bezzine, S.
    Purification and characterization of the first reptile pancreatic lipase: the turtle (2008), Pancreas, 36, 302-308.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 additional information not affected by EDTA and Ca2+ Testudo graeca nabeulensis
3.1.1.3 sodium deoxycholate completely inhibited at 4 mM sodium deoxycholate Testudo graeca nabeulensis
3.1.1.3 sodium taurodeoxycholate the inhibition is reversed by the addition of colipase Testudo graeca nabeulensis
3.1.1.3 tetrahydrolipstatine 40% residual activity in the absence of sodium deoxycholate, complete inhibition in the presence of 4 mM sodium deoxycholate Testudo graeca nabeulensis
3.1.1.3 Triton X-100 the activity of the enzyme dereases rapidly with a concentration of Triton X-100 greater than 0.3 mM Testudo graeca nabeulensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 45000
-
 SDS-PAGE Testudo graeca nabeulensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Testudo graeca nabeulensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 DEAE-cellulose column chromatography, Sephacryl S-200 gel filtration, and Mono-Q Sepharose column chromatography Testudo graeca nabeulensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 pancreas
-
 Testudo graeca nabeulensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 olive oil + H2O
-
 Testudo graeca nabeulensis ?
-
 ?
3.1.1.3 tributyrin + H2O
-
 Testudo graeca nabeulensis dibutyrin + butyrate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 glycerol ester hydrolase
-
 Testudo graeca nabeulensis
3.1.1.3 Pancreatic lipase
-
 Testudo graeca nabeulensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 37
-
-
 Testudo graeca nabeulensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 50
-
 the enzyme is inactivated at temperatures higher than 50°C after few min of incubation Testudo graeca nabeulensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8.2
-
-
 Testudo graeca nabeulensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 7 9 stable at pH 7-9, loses its activity at pH lower 4 after 1 h of incubation Testudo graeca nabeulensis