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Literature summary extracted from

  • Kaufmann, Y.; Todorova, V.K.; Luo, S.; Klimberg, V.S.
    Glutamine affects glutathione recycling enzymes in a DMBA-induced breast cancer model (2008), Nutr. Cancer, 60, 518-525.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.2 L-glutamine enhanced enzyme activity in jejunal mucosa Rattus norvegicus
3.5.1.2 L-glutamine enhanced enzyme activity in jejunal mucosa Rattus norvegicus
3.5.2.9 L-glutamine enhanced enzyme activity in jejunal mucosa of tumor-bearing rats Rattus norvegicus
6.3.2.2 L-glutamine enhanced enzyme activity in jejunal mucosa Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 L-glutamine inhibition of enzyme activity in tumor tissue Rattus norvegicus
3.5.1.2 L-glutamine inhibition of enzyme activity in tumor tissue Rattus norvegicus
3.5.2.9 L-glutamine inhibition of enzyme activity in tumor tissue Rattus norvegicus
6.3.2.2 L-glutamine inhibition of enzyme activity in tumor tissue Rattus norvegicus
6.3.2.2 additional information 7,12-dimethylbenz[a]anthracene does not affect GCS enzyme activity in gut tissue Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.2 Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.2 ATP + L-glutamate + L-cysteine Rattus norvegicus
-
 ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 additional information Rattus norvegicus tumor development in gut tissue does not affect GCS enzyme activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Rattus norvegicus P07314 Sprague-Dawley rats, female
-
3.5.1.2 Rattus norvegicus P13264 Sprague-Dawley rats, female
-
3.5.2.9 Rattus norvegicus
-
 Sprague-Dawley rats, female
-
6.3.2.2 Rattus norvegicus
-
 female Sprague-Dawley rats
-
6.3.2.2 Rattus norvegicus P19468 Sprague-Dawley rats, female
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 breast cancer cell
-
 Rattus norvegicus
-
2.3.2.2 jejunal mucosa
-
 Rattus norvegicus
-
3.5.1.2 breast cancer cell
-
 Rattus norvegicus
-
3.5.1.2 jejunal mucosa
-
 Rattus norvegicus
-
3.5.2.9 breast cancer cell
-
 Rattus norvegicus
-
3.5.2.9 jejunal mucosa
-
 Rattus norvegicus
-
6.3.2.2 breast cancer cell
-
 Rattus norvegicus
-
6.3.2.2 jejunal mucosa
-
 Rattus norvegicus
-
6.3.2.2 jejunum mucosa Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 12
-
 measured in jejunal mucosa in DMBA-treated rats wiht tumor Rattus norvegicus
2.3.2.2 15
-
 measured in jejunal mucosa in normal rats Rattus norvegicus
2.3.2.2 16
-
 measured in jejunal mucosa in DMBA-treated rats without tumor Rattus norvegicus
2.3.2.2 18
-
 measured in jejunal mucosa after treatment with dietary glutamine in DMBA-treated rats with tumor Rattus norvegicus
2.3.2.2 20
-
 measured in jejunal mucosa after treatment with dietary glutamine in normal rats Rattus norvegicus
2.3.2.2 25
-
 measured in jejunal mucosa after treatment with dietary glutamine in DMBA-treated rats without tumor Rattus norvegicus
2.3.2.2 45
-
 measured in tumor tissue after treatment with dietary glutamine Rattus norvegicus
2.3.2.2 80
-
 measured in tumor tissue Rattus norvegicus
3.5.1.2 additional information
-
 0.9 micromol/mg protein, measured in tumor tissue after treatment with dietary glutamine Rattus norvegicus
3.5.1.2 additional information
-
 1 micromol/mg protein, measured in jejunal mucosa in DMBA-treated rats wiht tumor Rattus norvegicus
3.5.1.2 additional information
-
 1,75 micromol/mg protein, measured in jejunal mucosa in normal rats Rattus norvegicus
3.5.1.2 additional information
-
 1.6 micromol/mg protein, measured in tumor tissue Rattus norvegicus
3.5.1.2 additional information
-
 2 micromol/mg protein, measured in jejunal mucosa after treatment with dietary glutamine in DMBA-treated rats with tumor Rattus norvegicus
3.5.1.2 additional information
-
 2.25 micromol/mg protein, measured in jejunal mucosa in DMBA-treated rats without tumor Rattus norvegicus
3.5.1.2 additional information
-
 3 micromol/mg protein, measured in jejunal mucosa after treatment with dietary glutamine in normal rats Rattus norvegicus
3.5.1.2 additional information
-
 5.25 micromol/mg protein, measured in jejunal mucosa after treatment with dietary glutamine in DMBA-treated rats without tumor Rattus norvegicus
3.5.2.9 1
-
 measured in tumor tissue after treatment with dietary glutamine Rattus norvegicus
3.5.2.9 1.25
-
 measured in jejunal mucosa in DMBA-teated rats with tumor Rattus norvegicus
3.5.2.9 2.5
-
 measured in tumor tissue Rattus norvegicus
3.5.2.9 2.91
-
 measured in jejunal mucosa in DMBA-treated rats without tumor Rattus norvegicus
3.5.2.9 3
-
 measured in jejunal mucosa after treatment with dietary glutamine in DMBA-treated rats without tumor Rattus norvegicus
3.5.2.9 3.75
-
 measured in jejunal mucosa in normal rats Rattus norvegicus
3.5.2.9 4.17
-
 measured in jejunal mucosa after treatment with dietary glutamine in normal rats Rattus norvegicus
3.5.2.9 4.33
-
 measured in jejunal mucosa after treatment with dietary glutamine in DMBA-treated rats with tumor Rattus norvegicus
6.3.2.2 additional information
-
 0.13 microg phosphate/mg protein, measured in tumor tissue after treatment with dietary glutamine Rattus norvegicus
6.3.2.2 additional information
-
 0.2 microg phosphate/mg protein, measured in tumor tissue Rattus norvegicus
6.3.2.2 additional information
-
 about 0.35 mmicrog phosphate/mg protein, measured in jejunal mucosa in normal rats and DMBA-treated rats with and without tumors Rattus norvegicus
6.3.2.2 additional information
-
 about 0.43 microg phosphate/mg protein, measured in jejunal mucosa after treatment with dietary glutamine in normal rats and in DMBA-treated rats with and without tumor Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 L-gamma-glutamyl-3-carboxy-4-nitroanilide + H2O assay at pH 8 Rattus norvegicus 3-carboxy-4-nitroaniline + L-glutamate
-
 ?
3.5.1.2 L-glutamine + H2O assay at pH 8 Rattus norvegicus L-glutamate + NH3
-
 ?
3.5.2.9 5-oxo-L-proline + ATP + 2 H2O assay at pH 7.2, 5 mM substrate, 4°C, 20 min Rattus norvegicus L-glutamate + phosphate + ADP
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine
-
 Rattus norvegicus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine assay at pH 8 Rattus norvegicus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 additional information tumor development in gut tissue does not affect GCS enzyme activity Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.2 gamma-glutamyltranspeptidase
-
 Rattus norvegicus
3.5.1.2 GLNase
-
 Rattus norvegicus
3.5.1.2 glutaminase
-
 Rattus norvegicus
3.5.2.9 5-oxo-L-prolinase
-
 Rattus norvegicus
3.5.2.9 OPase
-
 Rattus norvegicus
6.3.2.2 gamma-Glutamylcysteine synthetase
-
 Rattus norvegicus
6.3.2.2 GCS
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.9 4
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 8
-
 assay at Rattus norvegicus
3.5.1.2 8
-
 assay at Rattus norvegicus
3.5.2.9 7.2
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.2 ATP
-
 Rattus norvegicus