BRENDA - Enzyme Database show

A multisubstrate deoxyribonucleoside kinase from plants

Clausen, A.R.; Girandon, L.; Knecht, W.; Survery, S.; Andreasson, E.; Munch-Petersen, B.; Piskur, J.; Nucleic Acids Symp. Ser. 52, 489-490 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.145
upon expression in Escherichia coli, enzyme sensitizes cells against various nucleic acid analogs such as gemcitabine
Arabidopsis thaliana
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.145
Arabidopsis thaliana
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
2'-deoxyadenosine + ATP
specificity constant 33000 per s and M
694476
Arabidopsis thaliana
2'-deoxyadenosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxycytidine + ATP
specificity constant 18000 per s and M
694476
Arabidopsis thaliana
2'-deoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyguanosine + ATP
specificity constant 10000 per s and M
694476
Arabidopsis thaliana
2'-deoxyguanosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyuridine + ATP
specificity constant 410 per s and M
694476
Arabidopsis thaliana
2'-deoxyuridine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
bromovinyldeoxyuridine + ATP
specificity constant 930 per s and M
694476
Arabidopsis thaliana
bromovinyldeoxyuridine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
difluorodeoxycytidine + ATP
i.e. gemcitabine. Efficient in vivo phosphorylation, specificity constant 4800 per s and M
694476
Arabidopsis thaliana
difluorodeoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
additional information
no substrate: deoxythymidine
694476
Arabidopsis thaliana
?
-
-
-
-
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.145
upon expression in Escherichia coli, enzyme sensitizes cells against various nucleic acid analogs such as gemcitabine
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
2'-deoxyadenosine + ATP
specificity constant 33000 per s and M
694476
Arabidopsis thaliana
2'-deoxyadenosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxycytidine + ATP
specificity constant 18000 per s and M
694476
Arabidopsis thaliana
2'-deoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyguanosine + ATP
specificity constant 10000 per s and M
694476
Arabidopsis thaliana
2'-deoxyguanosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyuridine + ATP
specificity constant 410 per s and M
694476
Arabidopsis thaliana
2'-deoxyuridine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
bromovinyldeoxyuridine + ATP
specificity constant 930 per s and M
694476
Arabidopsis thaliana
bromovinyldeoxyuridine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
difluorodeoxycytidine + ATP
i.e. gemcitabine. Efficient in vivo phosphorylation, specificity constant 4800 per s and M
694476
Arabidopsis thaliana
difluorodeoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
additional information
no substrate: deoxythymidine
694476
Arabidopsis thaliana
?
-
-
-
-