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Literature summary extracted from

  • Morgan, R.D.; Bhatia, T.K.; Lovasco, L.; Davis, T.B.
    MmeI: a minimal Type II restriction-modification system that only modifies one DNA strand for host protection (2008), Nucleic Acids Res., 36, 6558-6570.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.21.4 S-adenosyl-L-methionine MmeI endonuclease activity requires S-adenosyl-L-methionine Methylophilus methylotrophus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 expressed in Escherichia coli strain ER2683 Methylophilus methylotrophus
3.1.21.4 expressed in Escherichia coli strain ER2683 Methylophilus methylotrophus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.4 additional information MmeI endonuclease is sensitive only to top strand methylation Methylophilus methylotrophus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.4 Mg2+ MmeI endonuclease activity requires magnesium ions Methylophilus methylotrophus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.72 105100
-
 calculated from amino acid sequence Methylophilus methylotrophus
3.1.21.4 105100
-
 calculated from amino acid sequence Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 additional information Methylophilus methylotrophus MmeI does not produce any detectable N4-cytosine methylation ?
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Methylophilus methylotrophus MmeI modifies only the adenine in the top strand, 5’-TCCRAC-3’, MmeI endonuclease activity is blocked by this top strand adenine methylation and is unaffected by methylation of the adenine in the complementary strand, 5’-GTYGGA-3' S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Methylophilus methylotrophus B2MU09
-
-
3.1.21.4 Methylophilus methylotrophus B2MU09
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 heparin Hyper D column chromatography, Source 15Q column chromatography, heparin TSK column chromatography, Source 15 S column chromatography, Superdex 75 gel filtration, and ceramic HTP column chromatography Methylophilus methylotrophus
3.1.21.4 heparin column chromatography, Source Q column chromatography, Source S column chromatography, Superdex 75 gel filtration, and ceramic HTP column chromatography Methylophilus methylotrophus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 additional information MmeI does not produce any detectable N4-cytosine methylation Methylophilus methylotrophus ?
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine MmeI modifies only the adenine in the top strand, 5’-TCCRAC-3’, MmeI endonuclease activity is blocked by this top strand adenine methylation and is unaffected by methylation of the adenine in the complementary strand, 5’-GTYGGA-3' Methylophilus methylotrophus S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
3.1.21.4 DNA + H2O MmeI cuts DNA two turns of the helix away from its asymmetric recognition sequence, 5'-TCCRACN20/N18-3', MmeI modifies only the adenine in the top strand, 5'-TCCRAC-3', MmeI endonuclease activity is blocked by this top strand adenine methylation and is unaffected by methylation of the adenine in the complementary strand, 5'-GTYGGA-3', MmeI methylates its recognition site following DNA cleavage Methylophilus methylotrophus double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 additional information the head-to-head configuration substrate, pUC19HH1, is digested both as closed circular DNA and linear DNA Methylophilus methylotrophus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 MmeI has both endonuclease and DNA methyltransferase activities Methylophilus methylotrophus
3.1.21.4 MmeI
-
 Methylophilus methylotrophus
3.1.21.4 type II restriction enzyme
-
 Methylophilus methylotrophus

General Information

EC Number General Information Comment Organism
2.1.1.72 physiological function MmeI DNA methylation prevents cleavage by MmeI Methylophilus methylotrophus