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Literature summary extracted from

  • Just, A.; Butter, F.; Trenkmann, M.; Heitkam, T.; Moerl, M.; Betat, H.
    A comparative analysis of two conserved motifs in bacterial poly(A) polymerase and CCA-adding enzyme (2008), Nucleic Acids Res., 36, 5212-5220.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.19 expressed in Escherichia coli BL21(DE3) or BL21(DE3)pLys cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.19 D212A mutant enzyme forms specifically incorporated A-residues with a fidelity comparable to that of the wild type enzyme Escherichia coli
2.7.7.19 E211A mutant enzyme forms specifically incorporated A-residues with a fidelity comparable to that of the wild type enzyme Escherichia coli
2.7.7.19 R215A the mutation leads to a dramatic loss in nucleotide specificity and the formation of poly(N) tails Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.19 Escherichia coli P0ABF1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.19 HiTrap chelating Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.19 ATP + RNA
-
 Escherichia coli diphosphate + RNA(A)n
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.19 PAP
-
 Escherichia coli
2.7.7.19 poly(A) polymerase
-
 Escherichia coli