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Literature summary extracted from

  • Hardeland, U.; Bentele, M.; Jiricny, J.; Schaer, P.
    The versatile thymine DNA-glycosylase: a comparative characterization of the human, Drosophila and fission yeast orthologs (2003), Nucleic Acids Res., 31, 2261-2271.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.27 gene Thd1, phylogenetic analysis, functional expression of a His-tagged truncated variant comprising residues 650M-1063N in Escherichia coli strain BL21 (DE3) Drosophila melanogaster
3.2.2.27 phylogenetic analysis, functional expression of Thp1p in Escherichia coli strain BL21 (DE3) Schizosaccharomyces pombe
3.2.2.29 gene Thd1, phylogenetic analysis, functional expression of a His-tagged truncated variant comprising residues 650M-1063N in Escherichia coli strain BL21 (DE3) Drosophila melanogaster
3.2.2.29 phylogenetic analysis, expression of His-tagged TDG in Escherichia coli strain BL21 (DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.27 additional information construction of a His-tagged truncated Thd1p variant comprising residues 650M-1063N from Escherichia coli strain BL21 (DE3) Drosophila melanogaster
3.2.2.29 additional information construction of a His-tagged truncated Thd1p variant comprising residues 650M-1063N from Escherichia coli strain BL21 (DE3) Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.27 37000
-
 x * 37000, recombinant enzyme, SDS-PAGE Schizosaccharomyces pombe
3.2.2.27 60000
-
 x * 60000, recombinant enzyme, SDS-PAGE Drosophila melanogaster
3.2.2.29 46000
-
 x * 46000, recombinant enzyme, SDS-PAGE Homo sapiens
3.2.2.29 60000
-
 x * 60000, recombinant enzyme, SDS-PAGE Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.27 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O Schizosaccharomyces pombe
-
 3,N4-ethenocytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.27 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O Drosophila melanogaster
-
 3,N4-ethenocytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.27 5-fluorouracil-mismatched double-stranded DNA + H2O Schizosaccharomyces pombe
-
 5-fluorouracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 5-fluorouracil-mismatched double-stranded DNA + H2O Drosophila melanogaster
-
 5-fluorouracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 5-methylcytosine-mismatched double-stranded DNA + H2O Drosophila melanogaster
-
 5-methylcytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.27 additional information Drosophila melanogaster 5-methylcytosine and thymine derivatives are processed with an appreciable efficiency only by the human and the Drosophila enzymes ?
-
 ?
3.2.2.27 thymine-mismatched double-stranded DNA + H2O Drosophila melanogaster
-
 thymine + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O Schizosaccharomyces pombe
-
 uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O Drosophila melanogaster
-
 uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.29 thymine-mismatched double-stranded DNA + H2O Homo sapiens the human enzyme excises thymine and uracil from G-T and G-U mismatches, respectively, and is therefore proposed to play a central role in the cellular defense against genetic mutation through spontaneous deamination of 5-methylcytosine and cytosine thymine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 uracil-mismatched double-stranded DNA + H2O Drosophila melanogaster
-
 uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.29 uracil-mismatched double-stranded DNA + H2O Homo sapiens the human enzyme excises thymine and uracil from G-T and G-U mismatches, respectively, and is therefore proposed to play a central role in the cellular defense against genetic mutation through spontaneous deamination of 5-methylcytosine and cytosine uracil + double-stranded DNA with abasic site
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.27 Drosophila melanogaster Q9V4D8
-
-
3.2.2.27 Schizosaccharomyces pombe O59825
-
-
3.2.2.29 Drosophila melanogaster Q9V4D8
-
-
3.2.2.29 Homo sapiens Q13569
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.27 recombinant Thp1p from Escherichia coli strain BL21 (DE3) Schizosaccharomyces pombe
3.2.2.29 recombinant His-tagged TDG from Escherichia coli strain BL21 (DE3) Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.27 additional information
-
 glycosylase activity of the recombinant enzyme, differential G-T processing, overview Schizosaccharomyces pombe
3.2.2.27 additional information
-
 glycosylase activity of the recombinant enzyme, differential G-T processing, overview Drosophila melanogaster
3.2.2.29 additional information
-
 glycosylase activity of the recombinant enzyme, differential G-T processing, overview Drosophila melanogaster
3.2.2.29 additional information
-
 glycosylase activity of the recombinant enzyme, differential G-T processing, overview Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.27 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O
-
 Schizosaccharomyces pombe 3,N4-ethenocytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.27 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O
-
 Drosophila melanogaster 3,N4-ethenocytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.27 5-fluorouracil-mismatched double-stranded DNA + H2O
-
 Schizosaccharomyces pombe 5-fluorouracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 5-fluorouracil-mismatched double-stranded DNA + H2O
-
 Drosophila melanogaster 5-fluorouracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 5-methylcytosine-mismatched double-stranded DNA + H2O
-
 Drosophila melanogaster 5-methylcytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.27 additional information 5-methylcytosine and thymine derivatives are processed with an appreciable efficiency only by the human and the Drosophila enzymes Drosophila melanogaster ?
-
 ?
3.2.2.27 additional information the enzyme shows a broad and species-specific substrate spectrum, substrate binding structure, overview. The common most efficiently processed substrates of all are uracil and 3,N4-ethenocytosine opposite guanine and 5-fluorouracil in any double-stranded DNA context, the enzyme is able to hydrolyze a non-damaged 5'-methylcytosine opposite G, and the double strand and mismatch dependency of the enzymes varies with the substrate Drosophila melanogaster ?
-
 ?
3.2.2.27 additional information the enzyme shows a broad and species-specific substrate spectrum, substrate binding structure, overview. The common most efficiently processed substrates of all are uracil and 3,N4-ethenocytosine opposite guanine and 5-fluorouracil in any double-stranded DNA context, the enzyme is able to hydrolyze a non-damaged 5'-methylcytosine opposite G, and the double strand and mismatch dependency of the enzymes varies with the substrate, G-T mismatch is no substrate for Thp1p. Thp1p shows little preference for mismatched substrate and processes uracil opposite A or in an ssDNA context with remarkable efficiency, overview Schizosaccharomyces pombe ?
-
 ?
3.2.2.27 thymine-mismatched double-stranded DNA + H2O
-
 Drosophila melanogaster thymine + double-stranded DNA with abasic site
-
 ?
3.2.2.27 thymine-mismatched double-stranded DNA + H2O G-T mismatch is only a poor substrate for Thd1p Drosophila melanogaster thymine + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O
-
 Schizosaccharomyces pombe uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O
-
 Drosophila melanogaster uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.29 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O
-
 Drosophila melanogaster 3,N4-ethenocytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-bromouracil-mismatched double-stranded DNA + H2O paired with guanine Homo sapiens 5-bromouracil + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-fluorouracil-mismatched double-stranded DNA + H2O
-
 Drosophila melanogaster 5-fluorouracil + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-fluorouracil-mismatched double-stranded DNA + H2O paired with guanine Homo sapiens 5-fluorouracil + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-hydroxymethyluracil-mismatched double-stranded DNA + H2O paired with guanine Homo sapiens 5-hydroxymethyluracil + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-methylcytosine-mismatched double-stranded DNA + H2O
-
 Drosophila melanogaster 5-methylcytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-methylcytosine-mismatched double-stranded DNA + H2O paired with guanine Homo sapiens 5-methylcytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 cytosine-mismatched double-stranded DNA + H2O paired with guanine Homo sapiens cytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 additional information human TDG removes the substrate pyrimidines 5-fluorouracil, 5-hydroxymethyluracil, 5-bromouracil, epsilonC and thymine paired with guanine with high relative efficiencies, substrate spectrum and substrate binding structure, overview Homo sapiens ?
-
 ?
3.2.2.29 additional information the enzyme shows a broad and species-specific substrate spectrum, substrate binding structure, overview. The common most efficiently processed substrates of all are uracil and 3,N4-ethenocytosine opposite guanine and 5-fluorouracil in any double-stranded DNA context, the enzyme is able to hydrolyze a non-damaged 5'-methylcytosine opposite G, and the double strand and mismatch dependency of the enzymes varies with the substrate Drosophila melanogaster ?
-
 ?
3.2.2.29 thymine-mismatched double-stranded DNA + H2O the human enzyme excises thymine and uracil from G-T and G-U mismatches, respectively, and is therefore proposed to play a central role in the cellular defense against genetic mutation through spontaneous deamination of 5-methylcytosine and cytosine Homo sapiens thymine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 thymine-mismatched double-stranded DNA + H2O G-T mismatch is only a poor substrate for Thd1p Drosophila melanogaster thymine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 thymine-mismatched double-stranded DNA + H2O paired with guanine Homo sapiens thymine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 uracil-mismatched double-stranded DNA + H2O
-
 Drosophila melanogaster uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.29 uracil-mismatched double-stranded DNA + H2O
-
 Homo sapiens uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.29 uracil-mismatched double-stranded DNA + H2O the human enzyme excises thymine and uracil from G-T and G-U mismatches, respectively, and is therefore proposed to play a central role in the cellular defense against genetic mutation through spontaneous deamination of 5-methylcytosine and cytosine Homo sapiens uracil + double-stranded DNA with abasic site
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.27 ? x * 37000, recombinant enzyme, SDS-PAGE Schizosaccharomyces pombe
3.2.2.27 ? x * 60000, recombinant enzyme, SDS-PAGE Drosophila melanogaster
3.2.2.29 ? x * 60000, recombinant enzyme, SDS-PAGE Drosophila melanogaster
3.2.2.29 ? x * 46000, recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.2.27 Thd1p
-
 Drosophila melanogaster
3.2.2.27 Thp1p
-
 Schizosaccharomyces pombe
3.2.2.29 More the enzyme belongs to the uracil-DNA glycosylase family Homo sapiens
3.2.2.29 TDG
-
 Homo sapiens
3.2.2.29 Thd1p
-
 Drosophila melanogaster
3.2.2.29 thymine DNA-glycosylase
-
 Drosophila melanogaster
3.2.2.29 thymine DNA-glycosylase
-
 Homo sapiens