EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.20.99.1 | arsenate | - |
Oryza sativa | |
1.20.99.1 | arsenate | dramatically increases expression of ACR2.1 | Oryza sativa | |
1.20.99.1 | additional information | ACR2.1 exhibits higher reductase activity than ACR2.2 | Oryza sativa | |
1.20.99.1 | additional information | ACR2.1 exhibits higher reductase activity than ACR2.2. Expression of ACR2.1 under conditions of phosphate starvation is faster than in the presence of phosphate | Oryza sativa |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.20.99.1 | expressed in an Escherichia coli strain in which the arsC gene is deleted and in a yeast (Saccharomyces cerevisiae) strain with a disrupted ACR2 gene | Oryza sativa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.20.99.1 | C71S | mutagenesis of cysteine residues in the putative active site HC(X) 5 R motif leads to nearly complete loss of both phosphatase and arsenate reductase activities | Oryza sativa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.20.99.1 | 0.1 | - |
reduced glutaredoxin | - |
Oryza sativa | |
1.20.99.1 | 0.2 | - |
reduced glutaredoxin | - |
Oryza sativa | |
1.20.99.1 | 9.2 | - |
arsenate | - |
Oryza sativa | |
1.20.99.1 | 9.9 | - |
arsenate | mutant C71S | Oryza sativa | |
1.20.99.1 | 12.2 | - |
arsenate | - |
Oryza sativa | |
1.20.99.1 | 24.4 | - |
arsenate | mutant C71S | Oryza sativa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.20.99.1 | 14330 | - |
sequence analysis | Oryza sativa |
1.20.99.1 | 14960 | - |
sequence analysis | Oryza sativa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.20.99.1 | Oryza sativa | Q10SX6 | - |
- |
1.20.99.1 | Oryza sativa | Q336V5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.20.99.1 | by nickel-nitrilotriacetic acid chromatography | Oryza sativa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.20.99.1 | root | ACR2.2 is observed only in roots following arsenate exposure | Oryza sativa | - |
1.20.99.1 | root | weak expression of ACR2.1 before addition of arsenate | Oryza sativa | - |
1.20.99.1 | shoot | - |
Oryza sativa | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.20.99.1 | arsenate + reduced glutaredoxin | yeast glutaredoxin ScGrx1p or Escherichia coli Grx2 | Oryza sativa | arsenite + acceptor | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.20.99.1 | ACR2.1 | - |
Oryza sativa |
1.20.99.1 | ACR2.2 | - |
Oryza sativa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.20.99.1 | 0.03 | - |
arsenate | mutant C70S | Oryza sativa | |
1.20.99.1 | 0.05 | - |
arsenate | mutant C71S | Oryza sativa | |
1.20.99.1 | 0.14 | - |
arsenate | - |
Oryza sativa | |
1.20.99.1 | 0.19 | - |
reduced glutaredoxin | - |
Oryza sativa | |
1.20.99.1 | 0.26 | - |
reduced glutaredoxin | - |
Oryza sativa | |
1.20.99.1 | 0.33 | - |
arsenate | - |
Oryza sativa |