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Literature summary extracted from

  • Vassiliou, A.G.; Fragoulis, E.G.; Vassilacopoulou, D.
    Detection, purification and identification of an endogenous inhibitor of L-dopa decarboxylase activity from human placenta (2008), Neurochem. Res., 34, 1089-1100.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.1.28 medicine the presence of a natural occurring ddc inhibitor in a peripheral tissue, may have interesting implications for future pharmacological treatment of neurodegenerative diseases such as inhibition of peripheral ddc, as well as, therapeutic approaches of neoplasias that are characterized by elevated levels of Ddc expression Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.28 annexin 5 endogenous inhibitor, identified and purified from human placenta presented in the membrane function, shows 30.4% inhibition by incubation of the human placenta samples in the presence of 0.34 unit ddc from mouse kidney homogenate. The inhibitor exhibits an optimum activity at 50 mM NaCl, pH 6.5, is heat labile and is deactivated by boiling. After incubation of the placental homogenate with proteinase K, inhibitory activity is partially abolished, suggesting that a population of inhibitor molecules is embedded in the membrane. Mus musculus
4.1.1.28 carbidopa non-competitive inhibitor Mus musculus
4.1.1.28 NSD-1015 non-competitive inhibitor Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.28 L-3,4-Dihydroxyphenylalanine Mus musculus
-
 Dopamine + CO2
-
 ?
4.1.1.28 L-5-hydroxytryptophan Mus musculus
-
 serotonin + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.28 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.28 kidney obtained from normal individuals just after sacrifice by carbon dioxide Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.28 L-3,4-Dihydroxyphenylalanine
-
 Mus musculus Dopamine + CO2
-
 ?
4.1.1.28 L-5-hydroxytryptophan
-
 Mus musculus serotonin + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.28 AADC
-
 Mus musculus
4.1.1.28 Aromatic L-amino acid decarboxylase
-
 Mus musculus
4.1.1.28 DDC
-
 Mus musculus
4.1.1.28 L-DOPA decarboxylase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.28 37
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.28 pyridoxal 5'-phosphate
-
 Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.28 additional information
-
 additional information Samples containing varying amounts of the purified molecule are incubated in the presence of 0.34 U Ddc from mouse kidney homogenate at room temperature for 15 min. This results in an inhibitor quantity-dependent increase of Ddc activity inhibition. The inhibitory activity reaches saturation, when 1.8 microg of purified inhibitor are added to the reaction. Samples containing 0.9 microg of the purified inhibitor are incubated in the presence of 0.34 U Ddc from mouse kidney homogenate for varying time periods at room temperature, prior to estimation of their inhibitory activity. The purified molecule causes a time-dependent inhibition of Ddc, while there is no inhibitory activity at 0 min. The inhibitory activity is seen to reach a maximum effect at 30 min, before declining. Mus musculus
4.1.1.28 0.000036
-
 annexin 5 inhibitor purified from mouse kidney homogenate ddc, classical non-competitive inhibitor Mus musculus