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Literature summary extracted from

  • Hirata, A.; Klein, B.J.; Murakami, K.S.
    The X-ray crystal structure of RNA polymerase from Archaea (2008), Nature, 451, 851-854.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.6 expression in Escherichia coli Saccharolobus solfataricus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.6 free enzyme and D/L subcomplex of archaeal RNAP, X-ray diffraction structure determination and analysis at 3.4 and 1.7 A resolution, respectively Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.6 Iron the enzyme possesses a unique Fe-S cluster Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.6 30000
-
 free enzyme, gel filtration Saccharolobus solfataricus
2.7.7.6 40600
-
 enzyme D/L subcomplex, gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn Saccharolobus solfataricus
-
 diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn Saccharolobus solfataricus P2
-
 diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Saccharolobus solfataricus Q980R2 and P58192 and Q980R1 and P95989 and Q980A3 and Q9UXD9 and Q980L5 and Q980Q9 and Q97ZJ9 and Q980K0 and Q980Z8 and Q97ZX7 and Q980B8 Q980R2: subunit A', P58192: subunit A'', Q980R1: subunit B, P95989: subunit D, Q980A3: subunit E', Q9UXD9: subunit F, Q980L5: subunit G, Q980Q9: subunit H, Q97ZJ9: subunit K, Q980K0: subunit L, Q980Z8: subunit N, Q97ZX7: subunit P, Q980B8: subunit 13
-
2.7.7.6 Saccharolobus solfataricus P2 Q980R2 and P58192 and Q980R1 and P95989 and Q980A3 and Q9UXD9 and Q980L5 and Q980Q9 and Q97ZJ9 and Q980K0 and Q980Z8 and Q97ZX7 and Q980B8 Q980R2: subunit A', P58192: subunit A'', Q980R1: subunit B, P95989: subunit D, Q980A3: subunit E', Q9UXD9: subunit F, Q980L5: subunit G, Q980Q9: subunit H, Q97ZJ9: subunit K, Q980K0: subunit L, Q980Z8: subunit N, Q97ZX7: subunit P, Q980B8: subunit 13
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6 recombinant enzymes partially from Escherichia coli Saccharolobus solfataricus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.6 nucleoside triphosphate + RNAn = diphosphate + RNAn+1 reaction mechanism Saccharolobus solfataricus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn
-
 Saccharolobus solfataricus diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn
-
 Saccharolobus solfataricus P2 diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.6 RNA polymerase II
-
 Saccharolobus solfataricus
2.7.7.6 RNAP II
-
 Saccharolobus solfataricus