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Literature summary extracted from

  • Dalhus, B.; Arvai, A.S.; Rosnes, I.; Olsen, O.E.; Backe, P.H.; Alseth, I.; Gao, H.; Cao, W.; Tainer, J.A.; Bjoras, M.
    Structures of endonuclease V with DNA reveal initiation of deaminated adenine repair (2009), Nat. Struct. Mol. Biol., 16, 138-143.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.7 expressed in Escherichia coli strain BL21 CodonPlus (DE3) RIL Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.21.7 EndoV in complex with a hypoxanthine lesion substrate and with product DNA, hanging drop vapour diffusion method, in 6-12% (w/v) MPEG 2k in 200 mM imidazole-matate buffer, pH 5.8-7.4 Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.7 D43A the mutant is unable to bind the Mg2+ cofactor Thermotoga maritima
3.1.21.7 Y80A the mutant is severely compromised in its ability to bind to DNA base lesions and the corresponding nicked product Thermotoga maritima
3.1.21.7 Y80F the mutant is similar to the wild type EndoV in its ability to bind to DNA base lesions and the corresponding nicked product Thermotoga maritima
3.1.21.7 Y80H the mutant is severely compromised in its ability to bind to DNA base lesions and the corresponding nicked product Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.7 Mg2+ contains one Mg2+ ion in the active site, which is required for activity Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.7 Thermotoga maritima Q9X2H9
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.7 HiTrap SP XL column chromatography Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.7 DNA + H2O EndoV hydrolyzes the second phosphodiester bond 3' of a deaminated base using Mg2+ as a cofactor Thermotoga maritima 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA
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 ?
3.1.21.7 additional information EndoV cleaves substrates with base mismatches and helical distortions, such as mismatch loops, hairpins and flap structures, EndoV cleaves mismatched base pairs preferentially at adenine and guanine purines, EndoV binds to cleaved mismatch base pair products with much lower affinity as compared to cleaved deaminated bases Thermotoga maritima ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.7 endonuclease V
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 Thermotoga maritima
3.1.21.7 EndoV
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 Thermotoga maritima