Literature summary extracted from
Sandigursky, M.; Faje, A.; Franklin, W.A.
Characterization of the full length uracil-DNA glycosylase in the extreme thermophile Thermotoga maritima (2001), Mutat. Res., 485, 187-195.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.2.27 |
gene tmudg, expression of His-tagged TMUDG in Escherichia coli strain BW310(DE3), TMUDG can substitute for the endogenous enzyme in Escherichia coli |
Thermotoga maritima |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.2.2.27 |
additional information |
weak product inhibition of TMUDG by uracil |
Thermotoga maritima |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.2.2.27 |
20578 |
- |
x * 20578, sequence calculation |
Thermotoga maritima |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.2.27 |
Thermotoga maritima |
- |
gene tmudg |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.2.27 |
recombinant His-tagged TMUDG from Escherichia coli strain BW310(DE3) by nickel affinity and ion exchange chromatography, tag cleavage by thrombin |
Thermotoga maritima |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.2.2.27 |
additional information |
optimal growth temperature of the organism is 80-90°C |
Thermotoga maritima |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.2.27 |
additional information |
the enzyme initiates base excision repair, BER, using a closed circular DNA substrate containing a unique U-G base pair |
Thermotoga maritima |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.2.27 |
? |
x * 20578, sequence calculation |
Thermotoga maritima |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.2.27 |
TMUDG |
- |
Thermotoga maritima |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.2.2.27 |
75 |
- |
the enzyme has an optimal activity temperature below the growth temperature of the organism of 80-90°C |
Thermotoga maritima |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
3.2.2.27 |
70 |
85 |
- |
Thermotoga maritima |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.2.2.27 |
additional information |
- |
heat inactivation kinetics of the truncated and full length TMUDG, overview |
Thermotoga maritima |
3.2.2.27 |
70 |
75 |
highly stable |
Thermotoga maritima |
3.2.2.27 |
75 |
- |
purified recombinant enzyme, 2.5 h, completely stable |
Thermotoga maritima |
3.2.2.27 |
85 |
- |
purified recombinant enzyme, after 15 min completely stable, after 30 min loss of 80% of activity |
Thermotoga maritima |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.2.2.27 |
7.5 |
- |
assay at |
Thermotoga maritima |