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Literature summary extracted from

  • Nakamura, N.; Morinaga, H.; Kikuchi, M.; Yonekura, S.-I.; Ishii1, N.; Yamamoto, K.; Yonei S.; Zhang, Q.-M.
    Cloning and characterization of uracil-DNA glycosylase and the biological consequences of the loss of its function in the nematode Caenorhabditis elegans (2008), Mutagenesis, 23, 407-413.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.27 gene ung-1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21 Caenorhabditis elegans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.27 additional information a Ung-1 defective mutant strain TM2862 shows no uracil excision activity, but the mutation in the ung-1 gene does not affect development, fertility and lifespan in Caenorhanditis elegans, suggesting the existence of backup enzyme Caenorhabditis elegans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.27 Bacillus subtilis Ung inhibitor
-
 Caenorhabditis elegans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.27 32000
-
 x * 32000, about, sequence calculation Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.27 uracil-mismatched double-stranded DNA + H2O Caenorhabditis elegans
-
 uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O Caenorhabditis elegans Bristol N2
-
 uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched single-stranded DNA + H2O Caenorhabditis elegans
-
 uracil + single-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched single-stranded DNA + H2O Caenorhabditis elegans Bristol N2
-
 uracil + single-stranded DNA with abasic site
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.27 Caenorhabditis elegans
-
-
-
3.2.2.27 Caenorhabditis elegans Bristol N2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.27 recombinant Ung-1 from Escherichia coli strain BL21 Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.27 additional information UNG-1 removes uracil from both U-G and U-A base pairs in DNA. It also removes uracil from single-stranded oligonucleotide substrate less efficiently than double-stranded oligonucleotide. The active site A is present between residues 116-137, active site B at residues 247-253. Aromatic residues Tyr125, Phe136 and His247 are involved in the stacking interaction with uracil Caenorhabditis elegans ?
-
 ?
3.2.2.27 additional information UNG-1 removes uracil from both U-G and U-A base pairs in DNA. It also removes uracil from single-stranded oligonucleotide substrate less efficiently than double-stranded oligonucleotide. The active site A is present between residues 116-137, active site B at residues 247-253. Aromatic residues Tyr125, Phe136 and His247 are involved in the stacking interaction with uracil Caenorhabditis elegans Bristol N2 ?
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O
-
 Caenorhabditis elegans uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O
-
 Caenorhabditis elegans Bristol N2 uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched single-stranded DNA + H2O
-
 Caenorhabditis elegans uracil + single-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched single-stranded DNA + H2O
-
 Caenorhabditis elegans Bristol N2 uracil + single-stranded DNA with abasic site
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.27 ? x * 32000, about, sequence calculation Caenorhabditis elegans

Synonyms

EC Number Synonyms Comment Organism
3.2.2.27 More UNG-1 belongs to the UNG family 1 Caenorhabditis elegans
3.2.2.27 UNG-1
-
 Caenorhabditis elegans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.27 26
-
 assay at Caenorhabditis elegans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.27 8
-
 assay at Caenorhabditis elegans