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Literature summary extracted from

  • Jia, J.; Arif, A.; Ray, P.S.; Fox, P.L.
    WHEP domains direct noncanonical function of glutamyl-prolyl tRNA synthetase in translational control of gene expression (2008), Mol. Cell, 29, 679-690.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.15 expression of C-terminally His-tagged, full-length EPRS Homo sapiens
6.1.1.17 expression of C-terminally His-tagged, full-length EPRS Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.17 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.15 Mg2+
-
 Homo sapiens
6.1.1.17 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.15 172000
-
-
 Homo sapiens
6.1.1.17 172000
-
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro Homo sapiens
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 additional information Homo sapiens the glutamyl-prolyl tRNA synthetase determines the specificity of the heterotetrameric GAIT complex suppressing translation of selected mRNAs in interferon-gamma-activated monocytic cells by binding to a 3' UTR element in target mRNAs, critical role of EPRS WHEP domains in targeting and regulating GAIT complex binding to RNA, mechanism, overview. The enzyme is essential in regulating inflammatory gene expression ?
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu Homo sapiens
-
 AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 additional information Homo sapiens the glutamyl-prolyl tRNA synthetase determines the specificity of the heterotetrameric GAIT complex suppressing translation of selected mRNAs in interferon-gamma-activated monocytic cells by binding to a 3' UTR element in target mRNAs, critical role of EPRS WHEP domains in targeting and regulating GAIT complex binding to RNA, mechanism, overview. The enzyme is essential in regulating inflammatory gene expression ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Homo sapiens
-
-
-
6.1.1.17 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.17 U-937 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro
-
 Homo sapiens AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 additional information the glutamyl-prolyl tRNA synthetase determines the specificity of the heterotetrameric GAIT complex suppressing translation of selected mRNAs in interferon-gamma-activated monocytic cells by binding to a 3' UTR element in target mRNAs, critical role of EPRS WHEP domains in targeting and regulating GAIT complex binding to RNA, mechanism, overview. The enzyme is essential in regulating inflammatory gene expression Homo sapiens ?
-
 ?
6.1.1.15 additional information the upstream WHEP pair of EPRS directs high-affinity binding to GAIT element-bearing mRNAs, while the overlapping, downstream pair binds NSAP1, which inhibits mRNA binding. Interaction of EPRS with ribosomal protein L13a and GAPDH induces a conformational witch that rescues mRNA binding and restores translational control, interaction analysis, overview Homo sapiens ?
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Homo sapiens AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 additional information the glutamyl-prolyl tRNA synthetase determines the specificity of the heterotetrameric GAIT complex suppressing translation of selected mRNAs in interferon-gamma-activated monocytic cells by binding to a 3' UTR element in target mRNAs, critical role of EPRS WHEP domains in targeting and regulating GAIT complex binding to RNA, mechanism, overview. The enzyme is essential in regulating inflammatory gene expression Homo sapiens ?
-
 ?
6.1.1.17 additional information the upstream WHEP pair of EPRS directs high-affinity binding to GAIT element-bearing mRNAs, while the overlapping, downstream pair binds NSAP1, which inhibits mRNA binding. Interaction of EPRS with ribosomal protein L13a and GAPDH induces a conformational witch that rescues mRNA binding and restores translational control, interaction analysis, overview Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.15 More the human EPRS consists of four major domains: an N-terminal elongation factor-1Bgamma-like domain, an ERS catalytic domain, a 300 amino acid linker domain containing three tandem WHEP domains, and a C-terminal PRS catalytic domain Homo sapiens
6.1.1.17 More the human EPRS consists of four major domains: an N-terminal elongation factor-1Bgamma-like domain, an ERS catalytic domain, a 300 amino acid linker domain containing three tandem WHEP domains, and a C-terminal PRS catalytic domain Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
6.1.1.15 glutamyl-prolyl tRNA synthetase
-
 Homo sapiens
6.1.1.15 More cf. EC 6.1.1.17 Homo sapiens
6.1.1.17 EPRS
-
 Homo sapiens
6.1.1.17 glutamyl-prolyl tRNA synthetase
-
 Homo sapiens
6.1.1.17 More cf. EC 6.1.1.15 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.15 ATP
-
 Homo sapiens
6.1.1.17 ATP
-
 Homo sapiens