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Literature summary extracted from

  • Bhomkar, P.; Upadhyay, C.P.; Saxena, M.; Muthusamy, A.; Shiva Prakash, N.; Pooggin, M.; Hohn, T.; Sarin, N.B.
    Salt stress alleviation in transgenic Vigna mungo L. Hepper (blackgram) by overexpression of the glyoxalase I gene using a novel Cestrum yellow leaf curling virus (CmYLCV) promoter (2008), Mol. Breed., 22, 169-181.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.5 methylglyoxal 10 mM, enhanced tolerance to toxic stress in transgenic Vigna mungo Brassica juncea
4.4.1.5 NaCl the transgenic and the untransformed plants are exposed to 100 mM NaCl. The transgenic plants survived whereas the untransformed control plants fail to survive Brassica juncea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 28000
-
Western blot analysis Brassica juncea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.5 methylglyoxal + glutathione Brassica juncea glyoxalase I is a member of the metallogltathione transferase superfamily and plays a critical role in detoxification of the cytotoxic methylglyoxal to S-D-lactoylglutathione via 1,2-hydrogen transfer S-((R)-lactoyl)glutathione
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Brassica juncea
-
gene gly I coding for glyoxalase I transformed into Vigna mungo L. Hepper using Agrobacterium tumefaciens, controlled by a novel constitutive Cestrum yellow leaf curling viral promotor
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.5 additional information young leaves of the transgenic plant Vigna mungo, transformed with gly1 from Brassica juncae using a transformation system via Agrobacterium tumefaciens, driven by a constitutive Cestrum yellow leaf curling viral promotor Brassica juncea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.5 additional information
-
the GLO1 activity of the transformed Vigna mungo plants is higher than in the untransformed control plants Brassica juncea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 methylglyoxal + glutathione glyoxalase I is a member of the metallogltathione transferase superfamily and plays a critical role in detoxification of the cytotoxic methylglyoxal to S-D-lactoylglutathione via 1,2-hydrogen transfer Brassica juncea S-((R)-lactoyl)glutathione
-
?
4.4.1.5 methylglyoxal + glutathione non-enzymatic formation of hemithioacetal from methylglyoxal and reduced glutathione Brassica juncea S-((R)-lactoyl)glutathione enzyme activity is measured spectrophotometrically as a function of thioester formation, S-((R)-lactoyl)glutathione, by measuring the rate of change of absorbance at 240 nm ?

Subunits

EC Number Subunits Comment Organism
4.4.1.5 ? x * 28000, Western blot analysis Brassica juncea

Synonyms

EC Number Synonyms Comment Organism
4.4.1.5 Gly I
-
Brassica juncea
4.4.1.5 glyoxalase I
-
Brassica juncea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.5 additional information
-
enzyme assay at room temperature Brassica juncea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.5 7.5
-
assay at Brassica juncea