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Literature summary extracted from

  • Quintana-Castro, R.; Diaz, P.; Valerio-Alfaro, G.; Garcia, H.S.; Oliart-Ros, R.
    Gene cloning, expression, and characterization of the Geobacillus thermoleovorans CCR11 thermoalkaliphilic lipase (2008), Mol. Biotechnol., 42, 75-83.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 expressed in Escherichia coli Geobacillus thermoleovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 40000
-
 mature lipase, SDS-PAGE Geobacillus thermoleovorans
3.1.1.3 42000
-
 mature lipase, calculated from amino acid sequence Geobacillus thermoleovorans
3.1.1.3 57000
-
 recombinant biotinylated enzyme, SDS-PAGE Geobacillus thermoleovorans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Geobacillus thermoleovorans Q0JRM8
-
-
3.1.1.3 Geobacillus thermoleovorans CCR11 Q0JRM8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl decanoate + H2O preferred substrate of the native mature enzyme Geobacillus thermoleovorans 4-nitrophenol + decanoate
-
 ?
3.1.1.3 4-nitrophenyl decanoate + H2O preferred substrate of the native mature enzyme Geobacillus thermoleovorans CCR11 4-nitrophenol + decanoate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Geobacillus thermoleovorans 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Geobacillus thermoleovorans CCR11 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl octanoate + H2O preferred substrate of the recombinant mature lipase Geobacillus thermoleovorans 4-nitrophenol + octanoate
-
 ?
3.1.1.3 4-nitrophenyl octanoate + H2O preferred substrate of the recombinant mature lipase Geobacillus thermoleovorans CCR11 4-nitrophenol + octanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 LipMatCCR11 lipase mature lipase protein Geobacillus thermoleovorans
3.1.1.3 triacylglycerol hydrolase
-
 Geobacillus thermoleovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 40
-
 recombinant mature lipase Geobacillus thermoleovorans
3.1.1.3 60
-
 native enzyme Geobacillus thermoleovorans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 30 70 native enzyme Geobacillus thermoleovorans
3.1.1.3 30 60 recombinant mature lipase Geobacillus thermoleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 9
-
 recombinant mature lipase Geobacillus thermoleovorans
3.1.1.3 9 10 native enzyme Geobacillus thermoleovorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 6 11 native enzyme Geobacillus thermoleovorans
3.1.1.3 7 10 recombinant mature lipase Geobacillus thermoleovorans