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Literature summary extracted from

  • Hatch, G.M.; Gu, Y.; Xu, F.Y.; Cizeau, J.; Neumann, S.; Park, J.S.; Loewen, S.; Mowat, M.R.
    StARD13(Dlc-2) RhoGap mediates ceramide activation of phosphatidylglycerolphosphate synthase and drug response in Chinese hamster ovary cells (2008), Mol. Biol. Cell, 19, 1083-1092.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.5 mitochondrion
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 Cricetulus griseus 5739
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.5 CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate Cricetulus griseus cardiolipin (phospholipid) biosynthesis, regulation by short chain cell-permeable ceramide (C2-Cer: N-acetylsphingosine) signalling and levels of active GTP-bound RhoA phosphatidylglycerophosphate + CMP
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.5 Cricetulus griseus Q9Z2Z7 chinese hamster
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.5 CHO-K1 cell chinese hamster ovary parental cell line (2.5fold induction of PGP synthase mRNA in response to N-acetylsphingosine-treatment, qRT-PCR) and etoposide-/N-acetylsphingosine-resistant cell line E91 (defect in ceramide signalling and RhoGAP activity, non-significant 1.2fold induction of PGP synthase mRNA in response to N-acetylsphingosine-treatment, qRT-PCR) Cricetulus griseus
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.5 additional information
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 135% increased activity in parental CHO-K1 cell line, stably expressing dominant-negative RhoA(S19N), N-acetylsphingosine-treated, mitochondrial fraction Cricetulus griseus
2.7.8.5 additional information
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 29% increased activity in CHO-K1 cell line E91, stably expressing dominant-negative RhoA(S19N), N-acetylsphingosine-treated, mitochondrial fraction Cricetulus griseus
2.7.8.5 additional information
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 64% increased activity in TNFalpha-treated (1 h, 30 nM) parental CHO-K1 cell line, mitochondrial fraction Cricetulus griseus
2.7.8.5 additional information
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 no change in activity in TNFalpha-treated (1 h, 30 nM) CHO-K1 cell line E91, mitochondrial fraction Cricetulus griseus
2.7.8.5 additional information
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 slighty decreased constitutive activity in CHO-K1 cell line E91, stably expressing dominant-negative RhoA(S19N), mitochondrial fraction Cricetulus griseus
2.7.8.5 0.000191
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 parental CHO-K1 cell line, mitochondrial fraction Cricetulus griseus
2.7.8.5 0.000192
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 CHO-K1 cell line E91, mitochondrial fraction Cricetulus griseus
2.7.8.5 0.000197
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 CHO-K1 cell line E91, N-acetylsphingosine-treated, no change in activity compared to untreated cells, mitochondrial fraction Cricetulus griseus
2.7.8.5 0.000299
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 or 76% increased activity in parental CHO-K1 cell line, N-acetylsphingosine-treated, mitochondrial fraction Cricetulus griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.5 CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate cardiolipin (phospholipid) biosynthesis, regulation by short chain cell-permeable ceramide (C2-Cer: N-acetylsphingosine) signalling and levels of active GTP-bound RhoA Cricetulus griseus phosphatidylglycerophosphate + CMP
-
 ?
2.7.8.5 CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate in vitro assay on mitochondrial fractions of cells grown in presence or absence of 30-50 microM N-acetylsphingosine Cricetulus griseus phosphatidylglycerophosphate + CMP
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.5 PGP synthase
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 Cricetulus griseus
2.7.8.5 phosphatidylglycerolphosphate synthase
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 Cricetulus griseus