EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.22.B62 | expressed in Hansenula polymorpha strain NCYC 495 | Fasciola hepatica |
3.4.22.B62 | the proenzyme is expressed in the methylotrophic yeast Hansenula polymorpha and the mature enzyme is obtained from the culture medium | Fasciola hepatica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.B62 | 0.0041 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | - |
Fasciola hepatica | |
3.4.22.B62 | 0.0041 | - |
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C | Fasciola hepatica | |
3.4.22.B62 | 0.0157 | - |
benzyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | - |
Fasciola hepatica | |
3.4.22.B62 | 0.0157 | - |
benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin | in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C | Fasciola hepatica | |
3.4.22.B62 | 0.0235 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | - |
Fasciola hepatica | |
3.4.22.B62 | 0.02353 | - |
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin | in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C | Fasciola hepatica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.B62 | additional information | Fasciola hepatica | cathepsin L3 is a main component of the juvenile secretory products and may participate in the tissue invasion process | ? | - |
? | |
3.4.22.B62 | additional information | Fasciola hepatica | does not cleave immunoglobulin | ? | - |
? | |
3.4.22.B62 | Type I collagen + H2O | Fasciola hepatica | CL3 exhibits the ability to degrade type I collagen efficiently at pH 5.5, 6.3 and 7.0 | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.B62 | Fasciola hepatica | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.B62 | - |
Fasciola hepatica |
3.4.22.B62 | HiTrap QXL column chromatography, gel filtration | Fasciola hepatica |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.22.B62 | juvenile | cathepsin L3 is a main component of the juvenile secretory products and may participate in invasion | Fasciola hepatica | - |
3.4.22.B62 | larva | CL3 is a main component of the juvenile secretory products | Fasciola hepatica | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.B62 | benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola hepatica | benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.22.B62 | benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin + H2O | - |
Fasciola hepatica | benzyloxycarbonyl-L-Val-L-Leu-L-Lys + 7-amino-4-methylcoumarin | - |
? | |
3.4.22.B62 | benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola hepatica | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.22.B62 | benzyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O | - |
Fasciola hepatica | benzyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin | - |
? | |
3.4.22.B62 | Collagen + H2O | FhCL3 efficiently cleaves type I collagen over different pH and temperature conditions | Fasciola hepatica | ? | - |
? | |
3.4.22.B62 | additional information | cathepsin L3 is a main component of the juvenile secretory products and may participate in the tissue invasion process | Fasciola hepatica | ? | - |
? | |
3.4.22.B62 | additional information | no cleavage of immunoglobulin | Fasciola hepatica | ? | - |
? | |
3.4.22.B62 | additional information | does not cleave immunoglobulin | Fasciola hepatica | ? | - |
? | |
3.4.22.B62 | additional information | cathepsin L3 has preference for proline in the selective P2 position | Fasciola hepatica | ? | - |
? | |
3.4.22.B62 | tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O | restricted substrate specificity with 70fold preference for tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin over benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin and benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin | Fasciola hepatica | ? | - |
? | |
3.4.22.B62 | tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O | 70fold preference for tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin over typical cathepsin substrates with hydrophobic or aliphatic residues in the S2 position | Fasciola hepatica | tosyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.22.B62 | Type I collagen + H2O | CL3 exhibits the ability to degrade type I collagen efficiently at pH 5.5, 6.3 and 7.0 | Fasciola hepatica | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.B62 | cathepsin L3 | - |
Fasciola hepatica |
3.4.22.B62 | CL3 | - |
Fasciola hepatica |
3.4.22.B62 | FhCL3 | - |
Fasciola hepatica |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.B62 | 0.33 | - |
benzyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | - |
Fasciola hepatica | |
3.4.22.B62 | 0.33 | - |
benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin | in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C | Fasciola hepatica | |
3.4.22.B62 | 0.39 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | - |
Fasciola hepatica | |
3.4.22.B62 | 0.39 | - |
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin | in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C | Fasciola hepatica | |
3.4.22.B62 | 6 | - |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin | - |
Fasciola hepatica | |
3.4.22.B62 | 6 | - |
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C | Fasciola hepatica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.B62 | 6 | - |
using benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin as substrate | Fasciola hepatica |
3.4.22.B62 | 7 | - |
- |
Fasciola hepatica |
3.4.22.B62 | 7 | - |
using tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin as substrate | Fasciola hepatica |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.B62 | 5 | 9 | CL3 has about 70% of the maximum activity at pH 5.0 and about 40% of the maximum activity at pH 9.0 using benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin as substrate | Fasciola hepatica |
3.4.22.B62 | 5.5 | 8 | CL3 has 35% of the maximum activity at pH 5.5 and about 70% of the maximum activity at pH 8.0 using tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin as substrate | Fasciola hepatica |
3.4.22.B62 | 6 | 8 | pH 6: about 70% of maximal activity, pH 8.0: abput 70% of maximal activity | Fasciola hepatica |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.B62 | 7 | - |
37°C, 50% loss of activity after 8 h, 64% loss of activity after 24 h | Fasciola hepatica |
3.4.22.B62 | 7 | - |
50% of the activity is conserved after 8 h incubation and 36% of it is retained after 24 h incubation at pH 7.0 and 37°C | Fasciola hepatica |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.B62 | 16.78 | - |
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin | in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C | Fasciola hepatica | |
3.4.22.B62 | 20.76 | - |
benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin | in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C | Fasciola hepatica | |
3.4.22.B62 | 1464 | - |
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C | Fasciola hepatica |