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Literature summary extracted from

  • Corvo, I.; Cancela, M.; Cappetta, M.; Pi-Denis, N.; Tort, J.F.; Roche, L.
    The major cathepsin L secreted by the invasive juvenile Fasciola hepatica prefers proline in the S2 subsite and can cleave collagen (2009), Mol. Biochem. Parasitol., 167, 41-47.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.B62 expressed in Hansenula polymorpha strain NCYC 495 Fasciola hepatica
3.4.22.B62 the proenzyme is expressed in the methylotrophic yeast Hansenula polymorpha and the mature enzyme is obtained from the culture medium Fasciola hepatica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.B62 0.0041
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
Fasciola hepatica
3.4.22.B62 0.0041
-
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C Fasciola hepatica
3.4.22.B62 0.0157
-
benzyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
-
Fasciola hepatica
3.4.22.B62 0.0157
-
benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C Fasciola hepatica
3.4.22.B62 0.0235
-
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
Fasciola hepatica
3.4.22.B62 0.02353
-
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C Fasciola hepatica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.B62 additional information Fasciola hepatica cathepsin L3 is a main component of the juvenile secretory products and may participate in the tissue invasion process ?
-
?
3.4.22.B62 additional information Fasciola hepatica does not cleave immunoglobulin ?
-
?
3.4.22.B62 Type I collagen + H2O Fasciola hepatica CL3 exhibits the ability to degrade type I collagen efficiently at pH 5.5, 6.3 and 7.0 ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B62 Fasciola hepatica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.B62
-
Fasciola hepatica
3.4.22.B62 HiTrap QXL column chromatography, gel filtration Fasciola hepatica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.B62 juvenile cathepsin L3 is a main component of the juvenile secretory products and may participate in invasion Fasciola hepatica
-
3.4.22.B62 larva CL3 is a main component of the juvenile secretory products Fasciola hepatica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B62 benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
-
Fasciola hepatica benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
?
3.4.22.B62 benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin + H2O
-
Fasciola hepatica benzyloxycarbonyl-L-Val-L-Leu-L-Lys + 7-amino-4-methylcoumarin
-
?
3.4.22.B62 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
Fasciola hepatica benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
?
3.4.22.B62 benzyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
Fasciola hepatica benzyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
?
3.4.22.B62 Collagen + H2O FhCL3 efficiently cleaves type I collagen over different pH and temperature conditions Fasciola hepatica ?
-
?
3.4.22.B62 additional information cathepsin L3 is a main component of the juvenile secretory products and may participate in the tissue invasion process Fasciola hepatica ?
-
?
3.4.22.B62 additional information no cleavage of immunoglobulin Fasciola hepatica ?
-
?
3.4.22.B62 additional information does not cleave immunoglobulin Fasciola hepatica ?
-
?
3.4.22.B62 additional information cathepsin L3 has preference for proline in the selective P2 position Fasciola hepatica ?
-
?
3.4.22.B62 tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O restricted substrate specificity with 70fold preference for tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin over benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin and benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin Fasciola hepatica ?
-
?
3.4.22.B62 tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O 70fold preference for tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin over typical cathepsin substrates with hydrophobic or aliphatic residues in the S2 position Fasciola hepatica tosyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
?
3.4.22.B62 Type I collagen + H2O CL3 exhibits the ability to degrade type I collagen efficiently at pH 5.5, 6.3 and 7.0 Fasciola hepatica ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.B62 cathepsin L3
-
Fasciola hepatica
3.4.22.B62 CL3
-
Fasciola hepatica
3.4.22.B62 FhCL3
-
Fasciola hepatica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.B62 0.33
-
benzyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
-
Fasciola hepatica
3.4.22.B62 0.33
-
benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C Fasciola hepatica
3.4.22.B62 0.39
-
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
Fasciola hepatica
3.4.22.B62 0.39
-
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C Fasciola hepatica
3.4.22.B62 6
-
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
Fasciola hepatica
3.4.22.B62 6
-
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C Fasciola hepatica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.B62 6
-
using benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin as substrate Fasciola hepatica
3.4.22.B62 7
-
-
Fasciola hepatica
3.4.22.B62 7
-
using tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin as substrate Fasciola hepatica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.B62 5 9 CL3 has about 70% of the maximum activity at pH 5.0 and about 40% of the maximum activity at pH 9.0 using benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin as substrate Fasciola hepatica
3.4.22.B62 5.5 8 CL3 has 35% of the maximum activity at pH 5.5 and about 70% of the maximum activity at pH 8.0 using tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin as substrate Fasciola hepatica
3.4.22.B62 6 8 pH 6: about 70% of maximal activity, pH 8.0: abput 70% of maximal activity Fasciola hepatica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.B62 7
-
37°C, 50% loss of activity after 8 h, 64% loss of activity after 24 h Fasciola hepatica
3.4.22.B62 7
-
50% of the activity is conserved after 8 h incubation and 36% of it is retained after 24 h incubation at pH 7.0 and 37°C Fasciola hepatica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.22.B62 16.78
-
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C Fasciola hepatica
3.4.22.B62 20.76
-
benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C Fasciola hepatica
3.4.22.B62 1464
-
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C Fasciola hepatica