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Literature summary extracted from

  • Wendler, A.; Irsch, T.; Rabbani, N.; Thornalley, P.J.; Krauth-Siegel, R.L.
    Glyoxalase II does not support methylglyoxal detoxification but serves as a general trypanothione thioesterase in African trypanosomes (2009), Mol. Biochem. Parasitol., 163, 19-27.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.6 cloning of RNA interference constructs for targeting GLX II in bloodstream and procyclic Trypanosoma brucei, cloning of the constructs for the deletion of the GLX II alleles in procyclic cells and cloning of the pHD1701/GLXIImyc2 plasmid for the overexpression of GLX II Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.6 0.109
-
 S-propionyltrypanothione
-
 Trypanosoma brucei
3.1.2.6 0.133
-
 S-hydroxyglutaryltrypanothione
-
 Trypanosoma brucei
3.1.2.6 0.144
-
 S-acetyltrypanothione
-
 Trypanosoma brucei
3.1.2.6 0.164
-
 S-bis-lactoyltrypanothione
-
 Trypanosoma brucei
3.1.2.6 0.195
-
 S-lactoylglutathionylspermidine
-
 Trypanosoma brucei
3.1.2.6 0.265
-
 S-glycolyltrypanothione
-
 Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.6 additional information Trypanosoma brucei recombinant glyoxalase II hydrolyzes the trypanothione-thioesters of methylglyoxal, glyoxal and 4,5-dioxovalerate, substrates of the classical glyoxalase system, with high efficiency ?
-
 ?
3.1.2.6 additional information Trypanosoma brucei The glyoxalase system, composed of glyoxalase I and II catalyzes the detoxification of reactive ketoaldehydes and thus protects cells from ketoaldehyde-mediated formation of advanced glycation end products. The main physiological substrate is the toxic and mutagenic methylglyoxal, which originates from triosephosphates during glycolysis as well as threonine catabolism. ?
-
 ?
3.1.2.6 S-acetyltrypanothione + H2O Trypanosoma brucei
-
 acetate + trypanothione
-
 ?
3.1.2.6 S-bis-lactoyltrypanothione + 2 H2O Trypanosoma brucei
-
 2 D-lactate + trypanothione
-
 ?
3.1.2.6 S-glycolyltrypanothione + H2O Trypanosoma brucei
-
 glycolate + trypanothione
-
 ?
3.1.2.6 S-hydroxyglutaryltrypanothione + H2O Trypanosoma brucei
-
 hydroxyglutarate + trypanothione + 2 H+
-
 ?
3.1.2.6 S-lactoylglutathionylspermidine + H2O Trypanosoma brucei
-
 ?
-
 ?
3.1.2.6 S-propionyltrypanothione + H2O Trypanosoma brucei
-
 propionate + trypanothione + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.6 Trypanosoma brucei Q581U6
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.6 449 cell culture-adapted bloodstream and procyclic Trypanosoma brucei of cell line 449, descendants of the strain Lister 427, stably transfected with pHD449 encoding the tetracycline repressor Trypanosoma brucei
-
3.1.2.6 cell culture for RNA interference experiments a cell line (427 1313 514) is employed that encodes a second tetracycline repressor and constitutively expresses a T7-polymerase Trypanosoma brucei
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.6 additional information
-
 after the onset of interfering RNAi, GLX II activity in extracts of bloodstream cells is decreased to about 20% when compared to the respective non-induced cells Trypanosoma brucei
3.1.2.6 additional information
-
 overexpression of GLX II has no apparent effect on Trypanosoma brucei Trypanosoma brucei
3.1.2.6 0.04
-
 clone B2 (pHD678/GLXII) plus tetracycline, GLX II activity in bloodstream Trypanosoma brucei subject to RNA interference Trypanosoma brucei
3.1.2.6 0.05
-
 clone B4 (p2T7/GLXII) plus tetracycline, GLX II activity in bloodstream Trypanosoma brucei subject to RNA interference Trypanosoma brucei
3.1.2.6 0.21
-
 wild-type minus tetracycine, GLX II activity in bloodstream Trypanosoma brucei subject to RNA interference Trypanosoma brucei
3.1.2.6 0.22
-
 clone B4 (p2T7/GLXII) minus tetracycline, GLX II activity in bloodstream Trypanosoma brucei subject to RNA interference Trypanosoma brucei
3.1.2.6 0.22
-
 clone K3 (pHD678) minus tetracycline, GLX II activity in bloodstream Trypanosoma brucei subject to RNA interference Trypanosoma brucei
3.1.2.6 0.23
-
 clone K3 (pHD678) plus tetracycline, GLX II activity in bloodstream Trypanosoma brucei subject to RNA interference Trypanosoma brucei
3.1.2.6 0.27
-
 clone B2 (pHD678/GLXII) minus tetracycline, GLX II activity in bloodstream Trypanosoma brucei subject to RNA interference Trypanosoma brucei
3.1.2.6 63
-
 S-hydroxyglutaryltrypanothione as substrate, kinetic parameters of recombinant enzyme are determined in 100 mM MOPS Trypanosoma brucei
3.1.2.6 73
-
 S-glycolyltrypanothione as substrate, kinetic parameters of recombinant enzyme are determined in 100 mM MOPS Trypanosoma brucei
3.1.2.6 92
-
 S-bis-lactoyltrypanothione as substrate, kinetic parameters of recombinant enzyme are determined in 100 mM MOPS Trypanosoma brucei
3.1.2.6 96
-
 S-propionyltrypanothione as substrate, kinetic parameters of recombinant enzyme are determined in 100 mM MOPS Trypanosoma brucei
3.1.2.6 115
-
 S-lactoylglutathionylspermidine as substrate, kinetic parameters of recombinant enzyme are determined in 100 mM MOPS Trypanosoma brucei
3.1.2.6 117
-
 S-acetyltrypanothione as substrate, kinetic parameters of recombinant enzyme are determined in 100 mM MOPS Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.6 additional information recombinant glyoxalase II hydrolyzes the trypanothione-thioesters of methylglyoxal, glyoxal and 4,5-dioxovalerate, substrates of the classical glyoxalase system, with high efficiency Trypanosoma brucei ?
-
 ?
3.1.2.6 additional information The glyoxalase system, composed of glyoxalase I and II catalyzes the detoxification of reactive ketoaldehydes and thus protects cells from ketoaldehyde-mediated formation of advanced glycation end products. The main physiological substrate is the toxic and mutagenic methylglyoxal, which originates from triosephosphates during glycolysis as well as threonine catabolism. Trypanosoma brucei ?
-
 ?
3.1.2.6 S-acetyltrypanothione + H2O
-
 Trypanosoma brucei acetate + trypanothione
-
 ?
3.1.2.6 S-bis-lactoyltrypanothione + 2 H2O
-
 Trypanosoma brucei 2 D-lactate + trypanothione
-
 ?
3.1.2.6 S-glycolyltrypanothione + H2O
-
 Trypanosoma brucei glycolate + trypanothione
-
 ?
3.1.2.6 S-hydroxyglutaryltrypanothione + H2O
-
 Trypanosoma brucei hydroxyglutarate + trypanothione + 2 H+
-
 ?
3.1.2.6 S-lactoylglutathionylspermidine + H2O
-
 Trypanosoma brucei ?
-
 ?
3.1.2.6 S-propionyltrypanothione + H2O
-
 Trypanosoma brucei propionate + trypanothione + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.6 Glx II
-
 Trypanosoma brucei
3.1.2.6 glyoxalase II
-
 Trypanosoma brucei
3.1.2.6 hydroxyacylglutathione hydrolase
-
 Trypanosoma brucei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.6 25
-
 assay at Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.6 7.2
-
 assay at Trypanosoma brucei