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Literature summary extracted from

  • Raverdy, S.; Foster, J.M.; Roopenian, E.; Carlow, C.K.
    The Wolbachia endosymbiont of Brugia malayi has an active pyruvate phosphate dikinase (2008), Mol. Biochem. Parasitol., 160, 163-166.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.9.1 drug development the enzyme is a good target for anti-parasite drug design Brugia malayi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.1 genomic analysis of the glycolytic/gluconeogenic pathway, optimization of C-terminally His-tagged PPDK expression in Escherichia coli as soluble protein, overview Brugia malayi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.9.1 96000
-
 x * 96000, recombinant His-tagged PPDK, SDS-PAGE Brugia malayi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.1 ATP + pyruvate + phosphate Brugia malayi
-
 AMP + phosphoenolpyruvate + diphosphate
-
 r
2.7.9.1 additional information Brugia malayi the filarial parasite Brugia malayi lacks pyruvate kinase and instead utilizes the enzyme pyruvate phosphate dikinase, PPDK. The reversible reaction catalyzed by PPDK occurs in three steps, where the outcome depends on the organism glycolysis and ATP formation, or PEP synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.1 Brugia malayi
-
 anendosymbiont, Wolbachia sp. subsp. Brugia malayi
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.1 soluble recombinant His-tagged PPDK from Escherichia coli by nickel affinity chromatography Brugia malayi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.1 ATP + pyruvate + phosphate
-
 Brugia malayi AMP + phosphoenolpyruvate + diphosphate
-
 r
2.7.9.1 additional information the filarial parasite Brugia malayi lacks pyruvate kinase and instead utilizes the enzyme pyruvate phosphate dikinase, PPDK. The reversible reaction catalyzed by PPDK occurs in three steps, where the outcome depends on the organism glycolysis and ATP formation, or PEP synthesis Brugia malayi ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.9.1 ? x * 96000, recombinant His-tagged PPDK, SDS-PAGE Brugia malayi

Synonyms

EC Number Synonyms Comment Organism
2.7.9.1 ATP:pyruvate, orthophosphate phosphotransferase
-
 Brugia malayi
2.7.9.1 PPDK
-
 Brugia malayi
2.7.9.1 pyruvate phosphate dikinase
-
 Brugia malayi

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.9.1 AMP reverse reaction Brugia malayi
2.7.9.1 ATP forward reaction Brugia malayi