Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mackie, G.A.; Coburn, G.A.; Miao, X.; Briant, D.J.; Prudhomme-Genereux, A.; Stickney, L.M.; Hankins, J.S.
    Preparation of the Escherichia coli RNase E protein and reconstitution of the RNA degradosome (2008), Methods Enzymol., 447, 199-213.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.12 Escherichia coli
-
-
-
3.1.26.12 Escherichia coli GM402 BL21(DE3)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.12 ammonium sulfate precipitation, Toyopearl column filtration chromatography, and Affi-Gel blue column chromatography Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.26.12 the diluted Rne is dialyzed against two changes of 100 volumes each of 60 mM NH4Cl, 0.1 mM dithiothreitol, and 10% (v/v) glycerol at 4°C for at least 20 h Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.12 additional information the C-terminus of this enzyme serves as a scaffold to which other components of the RNA degradosome bind including the phosphorolytic 3'-exonuclease, polynucleotide phosphorylase, the DEAD-box RNA helicase RhlB, and the glycolytic enzyme enolase. The DEAD-box RNA helicases CsdA and RhlE and the RNA binding protein Hfq may bind to RNase E in place of one or more of the prototypical components. Escherichia coli ?
-
 ?
3.1.26.12 additional information the C-terminus of this enzyme serves as a scaffold to which other components of the RNA degradosome bind including the phosphorolytic 3'-exonuclease, polynucleotide phosphorylase, the DEAD-box RNA helicase RhlB, and the glycolytic enzyme enolase. The DEAD-box RNA helicases CsdA and RhlE and the RNA binding protein Hfq may bind to RNase E in place of one or more of the prototypical components. Escherichia coli GM402 BL21(DE3) ?
-
 ?
3.1.26.12 RNA + H2O
-
 Escherichia coli ?
-
 ?
3.1.26.12 RNA + H2O
-
 Escherichia coli GM402 BL21(DE3) ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.26.12 RNase E
-
 Escherichia coli
3.1.26.12 Rne protein
-
 Escherichia coli