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Literature summary extracted from

  • Tsoupras, A.B.; Fragopoulou, E.; Nomikos, T.; Iatrou, C.; Antonopoulou, S.; Demopoulos, C.A.
    Characterization of the de novo biosynthetic enzyme of platelet activating factor, DDT-insensitive cholinephosphotransferase, of human mesangial cells (2007), Mediators Inflamm., 2007, 27683.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.2 bovine serum albumin specific activity increases in presence of 0.5-2 mg/ml bovine serum albumin, microsomal fraction of human mesangial cells Homo sapiens
2.7.8.2 dithiothreitol specific activity slightly increases at dithiothreitol (DTT) concentrations above 1 mM, optimum stimulation at 15 mM DTT, microsomal fraction of human mesangial cells Homo sapiens
2.7.8.2 EDTA maximum activation at 0.5 mM, in presence of 20 mM Mg2+ Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.2 Ca2+ 0.1 mM (14.1% activity left) or 1 mM (1.41% activity left), estimated in presence of 20 mM Mg2+, inhibition partially reduced in presence of 0.5 mM EDTA: 33.3% or 3.70% activity left for 0.1 mM or 1 mM Ca2+, respectively (100% activity: 20 mM Mg2+, 0.5 mM EDTA, no Ca2+) Homo sapiens
2.7.8.2 EDTA 10 mM, in presence of 20 mM Mg2+ Homo sapiens
2.7.8.2 INFalpha 250 inhibitory units/microl, 51% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
2.7.8.2 NaF sodium fluoride, 50 mM, 40% reduction in activity Homo sapiens
2.7.8.2 polar lipids from olive oil 5.2 ng/microl, 33% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
2.7.8.2 polar lipids from olive pomace 1.8 ng/microl, 48% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
2.7.8.2 polar lipids from sea bass 59 ng/microl, 45% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
2.7.8.2 polar lipids from sea bream 123 ng/microl, 17% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
2.7.8.2 resveratrol 100 microM, 39% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
2.7.8.2 rupatadine 20 ng/microl, 60% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
2.7.8.2 salicylic acid 250 ng/microl, 38% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
2.7.8.2 simvastatine 40 ng/microl, 92% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens
2.7.8.2 tinzaparin 0.25 inhibitory units/microl, 30% inhibition of enzyme activity in microsomal fraction of mesangial cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.2 0.0166
-
 CDP-choline optimum reaction conditions, in presence of 100 microM 1-O-alkyl-2-acetyl-sn-glycerol Homo sapiens
2.7.8.2 0.0593
-
 1-O-alkyl-2-acetyl-sn-glycerol optimum reaction conditions, in presence of 100 microM CDP-choline Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.2 microsome highest activity in microsomal fraction of mesangial cells Homo sapiens
-
-
2.7.8.2 additional information no activity detectable in cytoplasmic fraction of mesangial cells Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.2 Mg2+ 20 mM, maximum activation in presence of 0.5 mM EDTA Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.2 CDP-choline + 1-O-alkyl-2-acetyl-sn-glycerol Homo sapiens platelet activating factor (PAF) de novo biosynthesis CMP + 1-O-alkyl-2-acetyl-sn-glycerol 3-phosphocholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.2 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.2 mesangial cell HMC, immortilized by transfection with T-antigen of SV40 and Hras oncogene Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.2 additional information
-
 linear relationship between initial velocity and protein concentration in microsomal fraction of human mesangial cells up to 0.1 mg/ml Homo sapiens
2.7.8.2 additional information
-
 Pefabloc has no affect on enzyme activity, microsomal fraction of human mesangial cells Homo sapiens
2.7.8.2 0.0000003
-
 0.01 mM Mg2+, microsomal fraction of human mesangial cells Homo sapiens
2.7.8.2 0.0000012
-
 1 mM Mg2+, microsomal fraction of human mesangial cells Homo sapiens
2.7.8.2 0.0000036
-
 10 mM Mg2+, microsomal fraction of human mesangial cells Homo sapiens
2.7.8.2 0.00002
-
 mitochondrial fraction of human mesangial cells Homo sapiens
2.7.8.2 0.000067
-
 20 mM Mg2+, microsomal fraction of human mesangial cells Homo sapiens
2.7.8.2 0.0001
-
 homogenate of human mesangial cells Homo sapiens
2.7.8.2 0.00025
-
 20 mM Mg2+, 0.5 mM EDTA, microsomal fraction of human mesangial cells Homo sapiens
2.7.8.2 0.00036
-
 microsomal fraction of human mesangial cells, maximum activity in presence of 1 mg/ml bovine serum albumin, 15 mM dithiothreitol, 20 mM Mg2+, 0.5 mM EDTA Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.8.2 -20°C, microsomal fraction of human mesangial cells (HMC), 30 days Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.2 CDP-choline + 1-O-alkyl-2-acetyl-sn-glycerol platelet activating factor (PAF) de novo biosynthesis Homo sapiens CMP + 1-O-alkyl-2-acetyl-sn-glycerol 3-phosphocholine
-
 ?
2.7.8.2 CDP-choline + 1-O-alkyl-2-acetyl-sn-glycerol 37°C, 20 min, pH 8, in presence of 15 mM dithiothreitol, 5 mM EDTA, 20 mM MgCl2, 1 mg/ml bovine serum albumin Homo sapiens CMP + 1-O-alkyl-2-acetyl-sn-glycerol 3-phosphocholine analysis by thin-layer chromatography ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.2 DTT-insensitive CDP-choline: 1-alkyl-2-acetyl-sn-glycerol cholinephosphotransferase
-
 Homo sapiens
2.7.8.2 DTT-insensitive cholinephosphotransferase DTT: dithiothreitol Homo sapiens
2.7.8.2 PAF-CPT PAF: platelet activating factor, to be distinguished from PC-CPT, PC: phosphatidylcholine Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.2 37
-
 microsomal fraction of human mesangial cells (HMC), bell-shaped temperature activity profile Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.8.2 50
-
 20 min heating results in loss of 80% of activity, microsomal fraction of human mesangial cells (HMC) Homo sapiens
2.7.8.2 60
-
 20 min heating results in total loss of activity, microsomal fraction of human mesangial cells (HMC) Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.2 8
-
 100 mM Tris-HCl, bell-shaped pH activity profile Homo sapiens