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Literature summary extracted from

  • Liu, G.; Yue, L.; Chi, Z.; Yu, W.; Chi, Z.; Madzak, C.
    The Surface Display of the Alginate Lyase on the Cells of Yarrowia lipolytica for Hydrolysis of Alginate (2009), Mar. Biotechnol., 11, 619-626.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.2.3 molecular biology this is the first report that yeast cells displaying alginate lyase are used to produce different lengths of oligosaccharides from alginate Vibrio sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 gene of alginate lyase (AlyVI) from marine bacterium Vibrio is cloned into the multiple cloning site of the surface display vector pINA1317-YlCWP110 and expressed in cells of Yarrowia lipolytica Vibrio sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Vibrio sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate
-
 Vibrio sp. ?
-
 ?
4.2.2.3 poly-alpha-L-guluronic acid
-
 Vibrio sp. ?
-
 ?
4.2.2.3 poly-beta-D-mannuronate poly-beta-D-mannuronate can be much more easily hydrolyzed by the displayed alginate lyase than poly-alpha-L-guluronic acid and sodium alginate Vibrio sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase
-
 Vibrio sp.
4.2.2.3 AlyVI
-
 Vibrio sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 30
-
 assay at Vibrio sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7.2
-
 assay at Vibrio sp.
4.2.2.3 7.3
-
 maximal activity Vibrio sp.