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Literature summary extracted from

  • Han, Y.; Yang, B.; Zhang, F.; Miao, X.; Li, Z.
    Characterization of antifungal chitinase from marine Streptomyces sp. DA11 associated with South China Sea sponge Craniella australiensis (2009), Mar. Biotechnol., 11, 132-140.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 DNA and amino acid sequence determination and analysis Streptomyces sp. DA11

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Ba2+
-
 Streptomyces sp. DA11
3.2.1.14 EDTA
-
 Streptomyces sp. DA11
3.2.1.14 EGTA
-
 Streptomyces sp. DA11
3.2.1.14 Fe2+
-
 Streptomyces sp. DA11
3.2.1.14 SDS
-
 Streptomyces sp. DA11
3.2.1.14 theophylline
-
 Streptomyces sp. DA11
3.2.1.14 Urea
-
 Streptomyces sp. DA11

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 0.019
-
 colloidal chitin pH 8.0, 50°C Streptomyces sp. DA11
3.2.1.14 0.078
-
 powdered chitin pH 8.0, 50°C Streptomyces sp. DA11

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 Cu2+ activates Streptomyces sp. DA11
3.2.1.14 Mg2+ activates Streptomyces sp. DA11
3.2.1.14 Mn2+ activates Streptomyces sp. DA11
3.2.1.14 additional information optimal salinity for chitinase activity is 45 g psu Streptomyces sp. DA11

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 34000
-
 x * 34000, SDS-PAGE Streptomyces sp. DA11

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 additional information Streptomyces sp. DA11 the chitinase C shows antifungal activities against Aspergillus niger and Candida albicans. Streptomyces sp. strain DA11 is associated with South China sponge Craniella australiensis and might be involved in chitin degradation and antifungal defense of the sponge via its chitinase activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Streptomyces sp. DA11 B0LS89 fragment; marine strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native enzyme 6.15fold by ammonium sulfate fractionation, chitin affinity and anion exchange chromatography Streptomyces sp. DA11

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 2.95
-
 purified enzyme Streptomyces sp. DA11

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 colloidal chitin + H2O preferred substrate Streptomyces sp. DA11 ?
-
 ?
3.2.1.14 additional information the chitinase C shows antifungal activities against Aspergillus niger and Candida albicans. Streptomyces sp. strain DA11 is associated with South China sponge Craniella australiensis and might be involved in chitin degradation and antifungal defense of the sponge via its chitinase activity Streptomyces sp. DA11 ?
-
 ?
3.2.1.14 powdered chitin + H2O
-
 Streptomyces sp. DA11 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 34000, SDS-PAGE Streptomyces sp. DA11

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ChiC
-
 Streptomyces sp. DA11
3.2.1.14 chitinase C
-
 Streptomyces sp. DA11

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 50
-
-
 Streptomyces sp. DA11

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 8
-
-
 Streptomyces sp. DA11

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 6 9 stable, purified enzyme Streptomyces sp. DA11