Literature summary extracted from

Honda, Y.; Ohno, S.; Nakajin, S.
Leydig cells from neonatal pig testis abundantly express 11beta-hydroxysteroid dehydrogenase (11beta-HSD) type 2 and effectively inactivate cortisol to cortisone (2008), J. Steroid Biochem. Mol. Biol., 108, 91-101.

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1.1.B40	0.00038	-	cortisol	type 2 enzyme, 34°C, pH 7.4	Sus scrofa
1.1.1.B40	0.00136	-	cortisol	type 1 enzyme, 34°C, pH 7.4	Sus scrofa

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.1.1.B40	microsome	-	Sus scrofa	-	-

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.B40	Sus scrofa	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.1.1.B40	Leydig cell	of neonatal pig. High expression of 11beta-hydroxysteroid dehydrogenase type 2 mRNA, resulting in oxidation from cortisol to cortison, with no detection of the reduction reaction. Activity of type 2 enzyme is higher than the oxidation activity of the type 1 enzyme	Sus scrofa	-
1.1.1.B40	testis	of neonatal pig	Sus scrofa	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.B40	cortisol + NAD+	-	Sus scrofa	cortisone + NADH	-	?

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.B40	NAD+	-	Sus scrofa
1.1.1.B40	NADH	-	Sus scrofa

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Release 2023.1 (January 2023)