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Literature summary extracted from

  • Nilsson Lill, S.O.; Gao, J.; Waldrop, G.L.
    Molecular dynamics simulations of biotin carboxylase (2008), J. Phys. Chem. B, 112, 3149-3156.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.14 E288K inactive active-site mutant Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.14 Mg2+ coordinating interactions of active-site metal ions in wild-type and mutant enzymes, Mg2+ is bridged between the alpha- and gamma-phosphoryl group of ATP and coordinated to E288, E276, and a water molecule, detailed overview Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.14 ATP + biotin-carboxyl-carrier protein + CO2 Escherichia coli biotin carboxylase catalyzes the ATP-dependent carboxylation of biotin and is one component of the multienzyme complex acetyl-CoA carboxylase that catalyzes the first committed step in fatty acid synthesis ADP + phosphate + carboxybiotin-carboxyl-carrier protein
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 ?
6.3.4.14 additional information Escherichia coli the reaction mechanism of the acetyl-CoA carboxylase proceeds via two half-reactions: the first half-reaction is catalyzed by biotin carboxylase, the second half-reaction is catalyzed by carboxyltransferase ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.14 Escherichia coli P24182
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.14 ATP + biotin-carboxyl-carrier protein + CO2 biotin carboxylase catalyzes the ATP-dependent carboxylation of biotin and is one component of the multienzyme complex acetyl-CoA carboxylase that catalyzes the first committed step in fatty acid synthesis Escherichia coli ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?
6.3.4.14 ATP + biotin-carboxyl-carrier protein + CO2 in Gram-negative and Gram-positive bacteria biotin carboxylase, carboxyltransferase, and the biotin carboxyl carrier protein are separate proteins Escherichia coli ADP + phosphate + carboxybiotin-carboxyl-carrier protein
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 ?
6.3.4.14 additional information the reaction mechanism of the acetyl-CoA carboxylase proceeds via two half-reactions: the first half-reaction is catalyzed by biotin carboxylase, the second half-reaction is catalyzed by carboxyltransferase Escherichia coli ?
-
 ?
6.3.4.14 additional information molecular dynamics simulations of wild-type enzyme and active-site mutant E288K, overview Escherichia coli ?
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 ?

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.14 ATP ATP-dependent Escherichia coli