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Literature summary extracted from
- Structure of an archaeal homolog of the human protein complex Rpp21-Rpp29 that is a key core component for the assembly of active ribonuclease P (2008), J. Mol. Biol., 384, 652-662.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.26.5 | PhoRpp21 and PhoRpp29, hanging drop vapour diffusion method. using 50 mM Tris-HCl (pH 7.5) containing 50 mM sodium chloride, 200 mM potassium nitrate, and 20% w/v PEG 3350 | Pyrococcus horikoshii |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.26.5 | E47A | mutant shows activity similar to the wild type enzyme | Pyrococcus horikoshii |
| 3.1.26.5 | E73A | mutant shows reduced activity compared to the wild type enzyme | Pyrococcus horikoshii |
| 3.1.26.5 | F95A | mutant shows reduced activity compared to the wild type enzyme | Pyrococcus horikoshii |
| 3.1.26.5 | K121A | mutant shows activity similar to the wild type enzyme | Pyrococcus horikoshii |
| 3.1.26.5 | K122A | mutant shows activity similar to the wild type enzyme | Pyrococcus horikoshii |
| 3.1.26.5 | K90A | mutant shows strongly reduced activity compared to the wild type enzyme | Pyrococcus horikoshii |
| 3.1.26.5 | R115A | mutant shows reduced activity compared to the wild type enzyme | Pyrococcus horikoshii |
| 3.1.26.5 | R75A | mutant shows reduced activity compared to the wild type enzyme | Pyrococcus horikoshii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.26.5 | Pyrococcus horikoshii | O59425 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.26.5 | ribonucleoprotein | - |
Pyrococcus horikoshii |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.26.5 | SP-Sepharose column chromatography | Pyrococcus horikoshii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.5 | pre-tRNA precursor + H2O | - |
Pyrococcus horikoshii | tRNA + 5'-oligoribonucleotide | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.26.5 | RNase P | contains catalytic RNA and the RNase P proteins PhoRpp21 and PhoRpp29 | Pyrococcus horikoshii |
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Release 2023.1 (January 2023)
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